Anti-Mouse IL-7Ra/CD127 Rabbit Recombinant Antibody, PBS Only

• 

  FC experiment of mouse splenocytes using 98385-1-RR (same clone as 98385-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse IL-7Ra/CD127 Rabbit RecAb (98385-1-RR, Clone:242852B4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9). Cells were then stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed. This data was developed using the same antibody clone with 98385-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98385-1-RR (same clone as 98385-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse IL-7Ra/CD127 Rabbit RecAb (98385-1-RR, Clone:242852B4) (red) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were not fixed. This data was developed using the same antibody clone with 98385-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98385-1-RR (same clone as 98385-1-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse IL-7Ra/CD127 Rabbit RecAb (98385-1-RR, Clone:242852B4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.25 ug PE Anti-Mouse IL-7Ra/CD127 (A7R34) (PE-65093, Clone: A7R34). Cells were then stained with APC Anti-Mouse CD3 (17A2) (APC-65077, Clone: 17A2). Cells were not fixed. This data was developed using the same antibody clone with 98385-1-PBS in a different storage buffer formulation.

IL-7Ra/CD127 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98385-1-PBS

Clone No.242852B4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD127, Il7r, IL 7Ralpha, IL- 7Ralpha, IL-7 receptor subunit alpha

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Product Information

98385-1-PBS targets IL-7Ra/CD127 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1826

  Product name: Recombinant Mouse IL-7Ra/CD127 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 21-239 aa of NM_008372.4

  Sequence: ESGNAQDGDLEDADADDHSFWCHSQLEVDGSQHLLTCAFNDSDINTANLEFQICGALLRVKCLTLNKLQDIYFIKTSEFLLIGSSNICVKLGQKNLTCKNMAINTIVKAEAPSDLKVVYRKEANDFLVTFNAPHLKKKYLKKVKHDVAYRPARGESNWTHVSLFHTRTTIPQRKLRPKAMYEIKVRSIPHNDYFKGFWSEWSPSSTFETPEPKNQGGWD

• Full Name: interleukin 7 receptor
• Calculated Molecular Weight: 52 kDa
• GenBank Accession Number: NM_008372.4
• Gene Symbol: CD127
• Gene ID (NCBI): 16197
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16872
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD127, also known as IL-7R subunit alpha (IL-7Rα), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells (PMID: 8415665). IL-7R is a heterodimer composed of CD127 and IL-2Rγ (CD132), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15 (PMID: 8266077; 9862091). IL-7R plays critical roles in lymphocyte development and homeostasis (PMID: 26336149). CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP) (PMID: 10974032).