Tested Applications
| Positive WB detected in | ag14870 |
| Positive IF/ICC detected in | MOLT-4 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60269-1-Ig targets IL-10 in WB, IHC, IF/ICC, ELISA, Cell treatment applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, pig, rabbit, monkey, zebrafish |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14870 Product name: Recombinant human IL-10 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 19-178 aa of BC104252 Sequence: SPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN Predict reactive species |
| Full Name | interleukin 10 |
| Calculated Molecular Weight | 21 kDa |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | BC104252 |
| Gene Symbol | IL-10 |
| Gene ID (NCBI) | 3586 |
| RRID | AB_2881389 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P22301 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IL-10 antibody 60269-1-Ig | Download protocol |
| IHC protocol for IL-10 antibody 60269-1-Ig | Download protocol |
| WB protocol for IL-10 antibody 60269-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IL-10 Monoclonal antibody (catalog number 60269-1-Ig) has 225 citations. These appear in journals including Signal Transduction and Targeted Therapy, Immunity, ACS Nano, Advanced Science, Biomaterials, Journal of Nanobiotechnology, Theranostics, Frontiers in Immunology, International Immunopharmacology, Journal of Controlled Release, and Materials Today Bio. Research fields span immunology and inflammation, macrophage polarization, wound healing, bone regeneration, cancer, neuroinflammation, atherosclerosis, and tissue repair, with applications mainly in WB, IHC, IF, and ELISA.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Corynebacterium parakroppenstedtii secretes a novel glycolipid to promote the development of granulomatous lobular mastitis | ||
Immunity Expression of the readthrough transcript CiDRE in alveolar macrophages boosts SARS-CoV-2 susceptibility and promotes COVID-19 severity | ||
J Natl Cancer Cent S100A8 promotes tumor progression by inducing phenotypic polarization of microglia through the TLR4/IL-10 signaling pathway in glioma | ||
Bioact Mater Continuously released Zn2+ in 3D-printed PLGA/β-TCP/Zn scaffolds for bone defect repair by improving osteoinductive and anti-inflammatory properties | ||
Bioact Mater A bioactive composite hydrogel dressing that promotes healing of both acute and chronic diabetic skin wounds | ||
ACS Nano Tailored Extracellular Matrix-Biomimetic Coating Favors Tissue Healing by Modulating the Microenvironment. |
