Tested Applications
| Positive WB detected in | rat skeletal muscle tissue, Jurkat cells, mouse skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:300-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15102-1-AP targets IL-10RB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7445 Product name: Recombinant human IL-10RB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 18-225 aa of BC001903 Sequence: LGMVPPPENVRMNSVNFKNILQWESPAFAKGNLTFTAQYLSYRIFQDKCMNTTLTECDFSSLSKYGDHTLRVRAEFADEHSDWVNITFCPVDDTIIGPPGMQVEVLADSLHMRFLAPKIENEYETWTMKNVYNSWTYNVQYWKNGTDEKFQITPQYDFEVLRNLEPWTTYCVQVRGFLPDRNKAGEWSEPVCEQTTHDETVPSWMVAV Predict reactive species |
| Full Name | interleukin 10 receptor, beta |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 42-45 kDa |
| GenBank Accession Number | BC001903 |
| Gene Symbol | IL10RB |
| Gene ID (NCBI) | 3588 |
| RRID | AB_10638926 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08334 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Interleukin-10 (IL-10) is an anti-inflammatory cytokine that plays a critical role in maintaining immune system balance. IL-10 signals through a receptor complex consisting of two molecules of the IL-10R alpha-chain (IL-10RA) and two molecules of the accessory IL-10R beta-chain (IL-10RB) (PMID: 22236434). IL-10RA and IL-10RB are members of the type II cytokine receptor family. IL-10RB, also known as IL-10R2 or CDw210b, is the signaling subunit of the IL-10R complex and is constitutively expressed in a variety of cell types (PMID: 24507158). Binding of IL-10 to the IL-10R complex leads to JAK1- and TYK2-mediated phosphorylation of STAT3 (PMID: 11244051; 10433356). IL-10RB is also shared by several other cytokines, including IL22, IL-26, IFN-γ, IL-28A/B and IL29 (PMID: 22236434).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-10RB antibody 15102-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IL-10RB Polyclonal antibody (Cat# 15102-1-AP) has 6 citations published in journals including Nature Biotechnology, Advanced Healthcare Materials, BBA Molecular Basis of Disease, Frontiers in Bioscience, Journal of Alzheimer's Disease, and PLoS ONE. Research fields span RNA-protein interaction mapping, cancer immunotherapy, cardiac ischemia/reperfusion injury, exercise physiology and lipid metabolism, IL-10RB gene regulation in neurodegeneration, and myocardial infarction. Applications cited include WB and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Adv Healthc Mater Macrophage-Derived Nanosponges Adsorb Cytokines and Modulate Macrophage Polarization for Renal Cell Carcinoma Immunotherapy | ||
Biochim Biophys Acta Mol Basis Dis IL-22 ameliorated cardiomyocyte apoptosis in cardiac ischemia/reperfusion injury by blocking mitochondrial membrane potential decrease, inhibiting ROS and cytochrome C. | ||
Front Biosci (Landmark Ed) High-Intensity Interval Training Outperforms Moderate-Intensity Exercise in Hyperlipidemic ApoE⁻/⁻ Mice: A Molecular and Histological Comparison. | ||
PLoS One Endogenous interleukin-22 prevents cardiac rupture after myocardial infarction in mice |
