IL-15RA Monoclonal antibody

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  WB analysis of Raji using 60138-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60138-1-Ig (IL15RA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 60138-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 60138-1-Ig (IL15RA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 60138-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60138-1-Ig (IL15RA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

IL-15RA Monoclonal Antibody for WB, ELISA

Cat No. 60138-1-Ig

Clone No.5E3E1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

IL15RA, 5E3E1, CD215, IL 15 receptor subunit alpha, IL 15R alpha

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Tested Applications

• Positive WB detected in: Raji cells, Daudi cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60138-1-Ig targets IL-15RA in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10200

  Product name: Recombinant human IL-15RA protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-231 aa of BC107777

  Sequence: MSVEHADIWVKSYSLYSRERYICNSGFKRKAGTSSLTECVLNKATNVAHWTTPSLKCIRDPALVHQRPAPPSTVTTAGVTPQPESLSPSGKEPAASSPSSNNTAATTAAIVPGSQLMPSKSPSTGTTEISSHESSHGTPSQTTAKTWELTASASHQPPGVYPQGHSDTTVAISTSTVLLCGLSAVSLLACYLKSRQTPPLASVEMEAMEALPVTWGTSSRDEDLENCSHHL

• Full Name: interleukin 15 receptor, alpha
• Calculated Molecular Weight: 231 aa, 24 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC107777
• Gene Symbol: IL-15RA
• Gene ID (NCBI): 3601
• RRID: AB_2881352
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q13261
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897). The calculated molecular weight of full-length IL-15Rα is 28 kDa, IL-15Rα can be glycosylated, and larger apparent molecular weights of 55-65 kDa have been reported by some researches (PMID: 10480910; 15976182; 15671076; 17912462).