IL-16 Monoclonal antibody

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  WB analysis of Recombinant protein using 66145-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66145-1-Ig (IL-16 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 66145-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 66145-1-Ig against Human IL-16 was performed. The affinity constant is below 1 pM.

IL-16 Monoclonal Antibody for WB, ELISA

Cat No. 66145-1-Ig

Clone No.1C4C7

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA

Synonyms

IL16, 1C4C7, IL 16, interleukin-16, prIL 16

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Tested Applications

• Positive WB detected in: Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66145-1-Ig targets IL-16 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16466

  Product name: Recombinant human IL-16 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 154-454 aa of BC040272

  Sequence: MTKKSAAPTDRQPYSLCSNRKSLSQQLDCPAGKAAGTSRPTRSLSTAQLVQPSGGLQASVISNIVLMKGQAKGLGFSIVGGKDSIYGPIGIYVKTIFAGGAAAADGRLQEGDEILELNGESMAGLTHQDALQKFKQAKKGLLTLTVRTRLTAPPSLCSHLSPPLCRSLSSSTCITKDSSSFALESPSAPISTAKPNYRIMVEVSLQKEAGVGLGIGLCSVPYFQCISGIFVHTLSPGSVAHLDGRLRCGDEIVEISDSPVHCLTLNEVYTILSHCDPGPVSIIVSRHPDPQKEEMEAQEVK

• Full Name: interleukin 16 (lymphocyte chemoattractant factor)
• Calculated Molecular Weight: 130 aa, 15 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC040272
• Gene Symbol: IL-16
• Gene ID (NCBI): 3603
• RRID: AB_2881542
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14005
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL-16 is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. It is mainly produced by T lymphocytes but also by other immune cells, neuronal cells, fibroblasts and epithelial cells. The signaling process of this cytokine is mediated by CD4. IL-16 induces chemotaxis of CD4+ cells such as lymphocytes, eosinophils, and dendritic cells by ligating CD4 directly at a site distinct from other ligands. Among its multiple functions, IL-16 is a T cell chemoattractant involved in T helper cell inflammatory responses and the regulation of both T cell growth, and responsiveness to regulatory cytokines. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control.