Tested Applications
| Positive WB detected in | Recombinant protein | 
Recommended dilution
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66145-1-Ig targets IL-16 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human | 
| Host / Isotype | Mouse / IgG2b | 
| Class | Monoclonal | 
| Type | Antibody | 
| Immunogen | CatNo: Ag16466 Product name: Recombinant human IL-16 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 154-454 aa of BC040272 Sequence: MTKKSAAPTDRQPYSLCSNRKSLSQQLDCPAGKAAGTSRPTRSLSTAQLVQPSGGLQASVISNIVLMKGQAKGLGFSIVGGKDSIYGPIGIYVKTIFAGGAAAADGRLQEGDEILELNGESMAGLTHQDALQKFKQAKKGLLTLTVRTRLTAPPSLCSHLSPPLCRSLSSSTCITKDSSSFALESPSAPISTAKPNYRIMVEVSLQKEAGVGLGIGLCSVPYFQCISGIFVHTLSPGSVAHLDGRLRCGDEIVEISDSPVHCLTLNEVYTILSHCDPGPVSIIVSRHPDPQKEEMEAQEVKPredict reactive species | 
| Full Name | interleukin 16 (lymphocyte chemoattractant factor) | 
| Calculated Molecular Weight | 130 aa, 15 kDa | 
| Observed Molecular Weight | 37 kDa | 
| GenBank Accession Number | BC040272 | 
| Gene Symbol | IL-16 | 
| Gene ID (NCBI) | 3603 | 
| RRID | AB_2881542 | 
| Conjugate | Unconjugated | 
| Form | Liquid | 
| Purification Method | Protein A purification | 
| UNIPROT ID | Q14005 | 
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. | 
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. | 
Background Information
IL-16 is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. It is mainly produced by T lymphocytes but also by other immune cells, neuronal cells, fibroblasts and epithelial cells. The signaling process of this cytokine is mediated by CD4. IL-16 induces chemotaxis of CD4+ cells such as lymphocytes, eosinophils, and dendritic cells by ligating CD4 directly at a site distinct from other ligands. Among its multiple functions, IL-16 is a T cell chemoattractant involved in T helper cell inflammatory responses and the regulation of both T cell growth, and responsiveness to regulatory cytokines. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-16 antibody 66145-1-Ig | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 




