IL-17RB Polyclonal antibody

IL-17RB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 20673-1-AP

Synonyms

IL17RB, IL-17 receptor homolog 1, IL 17Rh1, IL 17RB, EVI27

---

Validation Data Gallery

• 

  WB analysis using 20673-1-AP

  Human testis tissue was subjected to SDS PAGE followed by western blot with 20673-1-AP (IL-17RB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 20673-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20673-1-AP (IL-17RB antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20673-1-AP (IL17RB antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20673-1-AP (IL17RB antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20673-1-AP (IL-17RB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 20673-1-AP (IL-17RB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20673-1-AP (IL17RB antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 20673-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 20673-1-AP (IL17RB antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 20673-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IL-17RB antibody (20673-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Tested Applications

• Positive WB detected in: human testis tissue, K-562 cells
• Positive IHC detected in: human kidney tissue, human breast cancer tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IF: See 3 publications below

Product Information

20673-1-AP targets IL-17RB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: interleukin 17 receptor B
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 56-60 kDa
• GenBank Accession Number: NM_018725
• Gene Symbol: IL-17RB
• Gene ID (NCBI): 55540
• RRID: AB_10700011
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL17RB, also named as CRL4, EVI27 and IL17BR, is a receptor for the proinflammatory cytokines IL17B and IL17E. IL17RB may play a role in controlling the growth and/or differentiation of hematopoietic cells. IL17RB has two isoforms with MW 56 kDa (isoform 1) and 32 kDa (isoform 2). The antibody is specific to IL17RB.

Publications

Species	Application	Title
human	WB	Nat Commun; Induction of IL-25 secretion from tumour-associated fibroblasts suppresses mammary tumour metastasis.; Authors - Shu-Yi Yin
human	WB,IF	Cell Rep; Schwann cell promotes macrophage recruitment through IL-17B/IL-17RB pathway in injured peripheral nerves; Authors - Yanju Huang
human	IF	Respir Res; IL-25 induces airway remodeling in asthma by orchestrating the phenotypic changes of epithelial cell and fibrocyte; Authors - Xiujuan Yao
human	WB,IF	Front Immunol; Interleukin-25-Mediated-IL-17RB Upregulation Promotes Cutaneous Wound Healing in Diabetic Mice by Improving Endothelial Cell Functions.; Authors - Fang Zhang
	WB	Arch Toxicol; Fluoride induces apoptosis and autophagy through the IL-17 signaling pathway in mice hepatocytes.; Authors - Yangfei Zhao
human	WB	mBio; Viral Mimicry of Interleukin-17A by SARS-CoV-2 ORF8.; Authors - Xin Wu