Tested Applications
| Positive WB detected in | human testis tissue, K-562 cells |
| Positive IHC detected in | human kidney tissue, human breast cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20673-1-AP targets IL-17RB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | interleukin 17 receptor B |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 56-60 kDa |
| GenBank Accession Number | NM_018725 |
| Gene Symbol | IL-17RB |
| Gene ID (NCBI) | 55540 |
| RRID | AB_10700011 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRM6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IL17RB, also named as CRL4, EVI27 and IL17BR, is a receptor for the proinflammatory cytokines IL17B and IL17E. IL17RB may play a role in controlling the growth and/or differentiation of hematopoietic cells. IL17RB has two isoforms with MW 56 kDa (isoform 1) and 32 kDa (isoform 2). The antibody is specific to IL17RB.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IL-17RB antibody 20673-1-AP | Download protocol |
| IHC protocol for IL-17RB antibody 20673-1-AP | Download protocol |
| WB protocol for IL-17RB antibody 20673-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IL-17RB Polyclonal antibody (Cat# 20673-1-AP) has 7 citations published in Nature Communications, Archives of Toxicology, Cell Reports, Frontiers in Immunology, Respiratory Research, mBio, and Pathological Research and Practice. Research fields span tumor metastasis suppression, hepatocyte apoptosis and autophagy, peripheral nerve injury and macrophage recruitment, diabetic wound healing, asthma-related airway remodeling, SARS-CoV-2 viral mimicry, and colorectal cancer immune infiltration.
| Species | Application | Title |
|---|---|---|
Nat Commun Induction of IL-25 secretion from tumour-associated fibroblasts suppresses mammary tumour metastasis. | ||
Arch Toxicol Fluoride induces apoptosis and autophagy through the IL-17 signaling pathway in mice hepatocytes. | ||
Cell Rep Schwann cell promotes macrophage recruitment through IL-17B/IL-17RB pathway in injured peripheral nerves | ||
Front Immunol Interleukin-25-Mediated-IL-17RB Upregulation Promotes Cutaneous Wound Healing in Diabetic Mice by Improving Endothelial Cell Functions. | ||
Respir Res IL-25 induces airway remodeling in asthma by orchestrating the phenotypic changes of epithelial cell and fibrocyte | ||
