IL-18BP Polyclonal antibody

IL-18BP Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14153-1-AP

Synonyms

IL 18BP, IL18BP, IL-18BP, IL18BPa, interleukin 18 binding protein, Tadekinig alfa

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Validation Data Gallery

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  WB analysis using 14153-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14153-1-AP (IL-18BP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14153-1-AP targets IL-18BP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-18BP fusion protein Ag5335
• Full Name: interleukin 18 binding protein
• Calculated Molecular Weight: 194 aa, 21 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC044215
• Gene Symbol: IL18BP
• Gene ID (NCBI): 10068
• RRID: AB_2918034
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IL18BP, also named as adekinig-alfa, has 3 isoforms (Isoform A 21kd; B 12kd and D 17kd). Isoform A binds to IL-18 and inhibits its activity. IL18BP functions as an inhibitor of the early TH1 cytokine response. Highly glycosylated of IL18BP will migrate to be 40kd. A 58kd protein is IL18BP crosslinked IL18.