IL18BP Recombinant antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP00670-4

  Sandwich ELISA standard curve of MP00670-4, Human IL18BP Recombinant Matched Antibody Pair - PBS only. 83692-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1147. 83692-5-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP00670-4

  Urine of eight individual healthy human donors was measured. The human IL18BP concentration of detected samples was determined to be 10.57 ng/mL with a range of 0.49 - 22.19 ng/mL.

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  Sample test of MP00670-4

  Serum of sixteen individual healthy human donors was measured. The human IL18BP concentration of detected samples was determined to be 2.06 ng/mL with a range of 0.68 - 3.14 ng/mL.

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  Sample test of MP00670-4

  Human peripheral blood mononuclear cells (PBMC) (1 x 10^6 cells/mL) were cultured in DMEM supplemented with 8% fetal bonive serum, 5μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human IL18BP.

IL18BP Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83692-2-PBS

Clone No.240711G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

IL 18BP, IL-18BP, IL18BPa, interleukin 18 binding protein, Interleukin-18-binding protein

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Product Information

83692-2-PBS targets IL18BP as part of a matched antibody pair:

MP00670-4: 83692-2-PBS capture and 83692-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: IL18BP fusion protein Eg1147
• Full Name: interleukin 18 binding protein
• Calculated Molecular Weight: 194 aa, 21 kDa
• GenBank Accession Number: BC044215
• Gene Symbol: IL-18BP
• Gene ID (NCBI): 10068
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95998
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.