IL18RAP Polyclonal antibody

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  WB analysis using 30587-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30587-1-AP (IL18RAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat spleen using 30587-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30587-1-AP (IL18RAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 30587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30587-1-AP (IL18RAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 30587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 30587-1-AP (IL18RAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 30587-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 30587-1-AP (IL18RAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IL18RAP Polyclonal Antibody for WB, IHC, ELISA

Cat No. 30587-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Accessory protein like, Accessory protein-like, ACPL, CD218 antigen-like family member B, CD218b

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Tested Applications

• Positive WB detected in: HL-60 cells, K-562 cells, RAW 264.7 cells, rat spleen tissue
• Positive IHC detected in: mouse spleen tissue, rat spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30587-1-AP targets IL18RAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL18RAP fusion protein Ag30327
• Full Name: interleukin 18 receptor accessory protein
• Calculated Molecular Weight: 599 aa, 68 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC069630
• Gene Symbol: IL18RAP
• Gene ID (NCBI): 8807
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95256
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-18 receptor accessory protein (IL18RAP, also known as IL1R7 and CD218b) is a crucial component of the IL-18 receptor complex. It enhances the binding activity of IL-18 to its receptor and plays a significant role in IL-18 signaling (PMID: 24842757). IL18RAP is related to the human tumor microenvironment and promotes various immune cell infiltration (PMID: 37698530).