Tested Applications
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13113-1-AP targets IL-36RN in IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3780 Product name: Recombinant human IL-1F5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-155 aa of BC024747 Sequence: MVLSGALCFRMKDSALKVLYLHNNQLLAGGLHAGKVIKGEEISVVPNRWLDASLSPVILGVQGGSQCLSCGVGQEPTLTLEPVNIMELYLGAKESKSFTFYRRDMGLTSSFESAAYPGWFLCTVPEADQPVRLTQLPENGGWNAPITDFYFQQCD Predict reactive species |
| Full Name | interleukin 1 family, member 5 (delta) |
| Calculated Molecular Weight | 155 aa, 17 kDa |
| GenBank Accession Number | BC024747 |
| Gene Symbol | IL-36RN |
| Gene ID (NCBI) | 26525 |
| RRID | AB_2877917 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBH0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IL36RN is a member of the interleukin 1 cytokine family. This cytokine was shown to specifically inhibit the activation of NF-kappaB induced by interleukin 1 family, member 6 (IL1F6). It inhibits the activity of interleukin-36 (IL36A,IL36B and IL36G) by binding to receptor IL1RL2 and preventing its association with the coreceptor IL1RAP for signaling.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IL-36RN antibody 13113-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IL-36RN Polyclonal antibody (Cat# 13113-1-AP) has 2 total citations. These appear in Experimental Dermatology (2025) and PLoS One (2021). The research fields span dermatology and immunology, specifically addressing severe dermatitis with metabolic wasting syndrome and barrier dysfunction in oral lichen planus. Both studies involve transcriptomic analysis, with one applying IHC validation in human tissue.
| Species | Application | Title |
|---|---|---|
Exp Dermatol Clinical Responses and Transcriptomic Analysis of Spesolimab in a Girl With Severe Dermatitis, Multiple Allergies and Metabolic Wasting Syndrome | ||
PLoS One Gene signatures associated with barrier dysfunction and infection in oral lichen planus identified by analysis of transcriptomic data |
