IL-1RAP Recombinant antibody, PBS Only (Capture)

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  Sandwich ELISA standard curve of MP00708-4

  Sandwich ELISA standard curve of MP00708-4, Human IL-1RAP Recombinant Matched Antibody Pair - PBS only. 98073-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0827. 83726-5-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL

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  Sample test of MP00708-4

  Plasma of six individual healthy human donors was measured. The human IL-1RAP concentration of detected samples was determined to be 266.79 pg/mL with a range of 13.79 - 1,302.80 pg/mL.

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  Sample test of MP00708-4

  Serum of six individual healthy human donors was measured. The human IL-1RAP concentration of detected samples was determined to be 21.67 pg/mL with a range of 7.89 - 42.37 pg/mL.

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  FC experiment of human PBMCs using 98073-1-RR (same clone as 98073-1-PBS)

  1x10^6 human PBMCs were stained with APC Anti-Human CD14 (26IC) (APC-65246, Clone: 26IC) and 0.25 ug Anti-Human IL-1RAP Rabbit Recombinant Antibody (98073-1-RR, Clone:240756A12), or Isotype Control. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98073-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 98073-1-RR (same clone as 98073-1-PBS)

  1x10^6 human PBMCs were stained with 0.25 ug Anti-Human IL-1RAP Rabbit Recombinant Antibody (98073-1-RR, Clone:240756A12)(red), or Isotype Control(blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98073-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98073-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98073-1-RR against Human IL-1RAP were performed. The affinity constant is 0.572 nM.

IL-1RAP Uni-rAb^TM Recombinant Antibody for FC, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 98073-1-PBS

Clone No.240756A12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

IL1RAP, IL 1RAcP, IL 1R3, IL 1R 3, EC:3.2.2.6

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Product Information

98073-1-PBS targets IL-1RAP as part of a matched antibody pair:

MP00708-4: 98073-1-PBS capture and 83726-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: interleukin 1 receptor accessory protein
• Calculated Molecular Weight: 65 kDa
• GenBank Accession Number: NM_002182.4
• Gene Symbol: IL1RAP
• Gene ID (NCBI): 3556
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9NPH3-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

IL-1RAP (Interleukin-1 receptor accessory protein), also known as IL-1RAcP, IL-1R-3, or IL-1R3, is a member of the interleukin-1 receptor family. IL-1RAP is composed of an extracellular region containing three Ig-like C2 type domains, a transmembrane region, and a cytoplasmic region with a TIR (Toll-IL-1-receptor) domain. IL-1RAP functions as a co-receptor for IL-1, IL-33, IL-36. It plays an essential role in the signaling of the IL-1 family cytokines such as IL-1, IL-33, and IL-36, as well as tyrosine kinases FLT3 and c-Kit. IL-1RAP can also exist in a soluble form.