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IL-28B Polyclonal antibody

IL-28B Polyclonal Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

IL28B, IL-28B/ IFNL3, IFN-lambda-3, IFNL4, IFNL3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 
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What's the best dilution for IL-28B in ?

Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24199-1-AP targets IL-28B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag21491

Product name: Recombinant human IL-28B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-196 aa of BC130316

Sequence: MTGDCMPVLVLMAAVLTVTGAVPVARLRGALPDARGCHIAQFKSLSPQELQAFKRAKDALEESLLLKDCKCRSRLFPRTWDLRQLQVRERPVALEAELALTLKVLEATADTDPALGDVLDQPLHTLHHILSQLRACIQPQPTAGPRTRGRLHHWLHRLQEAPKKESPGCLEASVTFNLFRLLTRDLNCVASGDLCV

Predict reactive species
Full Name interleukin 28B (interferon, lambda 3)
Calculated Molecular Weight 196 aa, 22 kDa
GenBank Accession NumberBC130316
Gene Symbol IL-28B
Gene ID (NCBI) 282617
RRIDAB_2879452
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZI9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for IL-28B antibody 24199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The IL-28B Polyclonal antibody (Cat# 24199-1-AP) has 1 total citation. The cited publication appeared in BMC Molecular and Cellular Biology (IF: 3), published in February 2020. The study investigated a minicircle DNA vector expressing interferon-lambda-3 for inhibiting hepatitis B virus replication in hepatocyte-derived cells, placing the research field within virology and liver biology. The antibody was used for Western blot analysis on human samples.

SpeciesApplicationTitle
humanWB

BMC Mol Cell Biol

Minicircle DNA vector expressing interferon-lambda-3 inhibits hepatitis B virus replication and expression in hepatocyte-derived cell line.

Authors - Xiaoyan Guo
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