Tested Applications
| Positive WB detected in | NK-92 cells |
| Positive IHC detected in | mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
13602-1-AP targets CD122/IL-2RB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3929 Product name: Recombinant human IL-2RB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 270-551 aa of BC025691 Sequence: TGPWLKKVLKCNTPDPSKFFSQLSSEHGGDVQKWLSSPFPSSSFSPGGLAPEISPLEVLERDKVTQLLLQQDKVPEPASLSSNHSLTSCFTNQGYFFFHLPDALEIEACQVYFTYDPYSEEDPDEGVAGAPTGSSPQPLQPLSGEDDAYCTFPSRDDLLLFSPSLLGGPSPPSTAPGGSGAGEERMPPSLQERVPRDWDPQPLGPPTPGVPDLVDFQPPPELVLREAGEEVPDAGPREGVSFPWSRPPGQGEFRALNARLPLNTDAYLSLQELQGQDPTHLV Predict reactive species |
| Full Name | interleukin 2 receptor, beta |
| Calculated Molecular Weight | 551 aa, 61 kDa |
| Observed Molecular Weight | 70-85 kDa |
| GenBank Accession Number | BC025691 |
| Gene Symbol | IL2RB |
| Gene ID (NCBI) | 3560 |
| ENSEMBL Gene ID | ENSG00000100385 |
| RRID | AB_10641033 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14784 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interleukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. The beta subunit (IL2RB, also known as CD122 and p75) is involved in receptor mediated endocytosis and transduces the mitogenic signals of nterleukin 2.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD122/IL-2RB antibody 13602-1-AP | Download protocol |
| WB protocol for CD122/IL-2RB antibody 13602-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Activation of IL-2/IL-2R pathway by Hedyotis diffusa polysaccharide improves immunotherapy in colorectal cancer | ||
Allergol Immunopathol (Madr) IL2RB affects Th1/Th2 and Th17 responses of peripheral blood mononuclear cells from septic patients | ||
Ocul Surf Conditional deletion of CD25 in the corneal epithelium reveals sex differences in barrier disruption | ||
J Dermatol Sci Exosomes containing miR-1469 regulate natural killer cells by targeting CD122 in non-segmental vitiligo | ||
ACS Omega Vanillic Acid Protects PC12 Cells from Corticosterone-Induced Neurotoxicity via Regulating Immune and Metabolic Dysregulation Based on Computational Metabolomics |
