IL-2RG Polyclonal antibody

IL-2RG Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 27377-1-AP

Synonyms

CD132, IL 2 receptor subunit gamma, IL 2R subunit gamma, IL 2RG, IL2RG, IL-2RG, IMD4, p64, SCIDX, SCIDX1

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Validation Data Gallery

• 

  WB analysis of Jurkat using 27377-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27377-1-AP (IL2RG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 27377-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27377-1-AP (IL2RG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 27377-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 27377-1-AP (IL2RG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, Raji cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27377-1-AP is IL-2RG Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-2RG fusion protein Ag26388
• Full Name: interleukin 2 receptor, gamma (severe combined immunodeficiency)
• Calculated Molecular Weight: 369 aa, 42 kDa
• Observed Molecular Weight: 64-70 kDa
• GenBank Accession Number: BC014972
• Gene Symbol: IL2RG
• Gene ID (NCBI): 3561
• RRID: AB_2880857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The interleukin-2 receptor (IL-2R) is a heterotrimeric receptor consisting of three subunits, alpha (IL2RA), beta (IL2RB), and gamma (IL2RG). IL2RG, also known as CD132, is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in the gene of IL2RG cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.