IL-32 Polyclonal antibody

• Featured Product
• KD/KO Validated

IL-32 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 11079-1-AP

Synonyms

IL 32, IL 32alpha, IL 32beta, IL 32delta, IL 32gamma, IL32, IL-32, interleukin 32, Natural killer cells protein 4, NK4, TAIF, TAIFa, TAIFb, TAIFc, TAIFd

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Validation Data Gallery

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  IHC staining of human lung cancer using 11079-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11079-1-AP (IL-32 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11079-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11079-1-AP (IL-32 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11079-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11079-1-AP (IL-32 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11079-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11079-1-AP (IL-32 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• ELISA: See 1 publications below

Product Information

11079-1-AP targets IL-32 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IL-32 fusion protein Ag1484
• Full Name: interleukin 32
• Calculated Molecular Weight: 27 kDa
• GenBank Accession Number: BC009401
• Gene Symbol: IL32
• Gene ID (NCBI): 9235
• RRID: AB_2877745
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin 32 (IL32) is a member of the cytokine family. IL32 contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. IL32 is a predominantly intracellular proinflammatory cytokine produced by epithelial cells, monocytes, T-lymphocytes and natural killer cells, and is involved in inflammation and cancer development. IL32 is able to induce the release a variety of pro-inflammatory cytokines such as IL8, tumor necrosis factor-α (TNFα) and IL6. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2.

Publications

Species	Application	Title
human	WB	J Cell Mol Med; MicroRNA-29b-3p suppresses oral squamous cell carcinoma cell migration and invasion via IL32/AKT signalling pathway.; Authors - Jianya He
human	IHC	J Hepatocell Carcinoma; Identification and Validation of Novel Biomarkers for Hepatocellular Carcinoma, Liver Fibrosis/Cirrhosis and Chronic Hepatitis B via Transcriptome Sequencing Technology.; Authors - Dandan Zhao
human,mouse	IHC,IF	Front Pharmacol; Targeting TIGIT Inhibits Bladder Cancer Metastasis Through Suppressing IL-32.; Authors - Kang Wu
human	WB,IHC	Oncol Lett; Interleukin-32 regulates downstream molecules and promotes the invasion of pancreatic cancer cells.; Authors - Kohji Takagi ; KD Validated
human	WB,IF	Oncol Lett; Establishment of highly invasive pancreatic cancer cell lines and the expression of IL-32.; Authors - Kohji Takagi
human	WB	Biomed Res Int; IL-32 Promotes the Radiosensitivity of Esophageal Squamous Cell Carcinoma Cell through STAT3 Pathway.; Authors - Zhiyu Ma