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- KD/KO Validated
IL-6 Polyclonal antibody
IL-6 Polyclonal Antibody for FC, WB, ELISA
Cat no : 21865-1-AP
|Positive WB detected in
|LPS and Protein transport inhibitor treated HUVEC cells
|Positive FC detected in
|LPS and Brefeldin A treated HUVEC cells
|Western Blot (WB)
|WB : 1:500-1:2000
|Flow Cytometry (FC)
|FC : 0.50 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
21865-1-AP targets IL-6 in WB, Neutralization, IHC, FC, Cell treatment, ELISA applications and shows reactivity with human samples.
|human, goat, pig, canine
|Host / Isotype
|Rabbit / IgG
|IL-6 fusion protein Ag10565
|interleukin 6 (interferon, beta 2)
|Calculated molecular weight
|212 aa, 24 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Udesh (Verified Customer) (04-19-2023)
Lowest most band corresponds to IL-6
Elizabeth (Verified Customer) (12-03-2018)