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IL-6 Monoclonal antibody

IL-6 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A3B4

Cat no : 66146-1-Ig

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HSF, Hybridoma growth factor, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6



Tested Applications

Positive WB detected inU-87 MG cells, Recombinant protein, Jurkat cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66146-1-Ig is IL-6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IL-6 fusion protein Ag10646
Full Name interleukin 6 (interferon, beta 2)
Calculated molecular weight 212 aa, 24 kDa
Observed molecular weight24 kDa
GenBank accession numberBC015511
Gene symbol IL6
Gene ID (NCBI) 3569
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Protocols

Product Specific Protocols
WB protocol for IL-6 antibody 66146-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

A stress-induced cilium-to-PML-NB route drives senescence initiation

Authors - Xiaoyu Ma
mouseIF

Nat Commun

Catalytically potent and selective clusterzymes for modulation of neuroinflammation through single-atom substitutions.

Authors - Haile Liu
mouseWB

Acta Biomater

Porous composite hydrogels with improved MSC survival for robust epithelial sealing around implants and M2 macrophage polarization

Authors - Yongzheng Li
mouseIF

J Hazard Mater

Polystyrene microplastics induced male reproductive toxicity in mice.

Authors - Haibo Jin
mouseIF

J Hazard Mater

Folic acid targets splenic extramedullary hemopoiesis to attenuate carbon black-induced coagulation-thrombosis potential.

Authors - Qingtao Meng
ratWB,IHC,IF

Bioact Mater

Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.

Authors - Liwen Luo