IL-6 Monoclonal antibody

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  WB analysis using 66146-1-Ig

  Non-treated and protein transport inhibitor treated U-87 MG cells were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66146-1-Ig

  Non-treated, LPS and protein transport inhibitor treated HUVEC cells were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66146-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 66146-1-Ig

  Recombinant protein were subjected to SDS PAGE followed by western blot with 66146-1-Ig (IL-6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HUVEC using 66146-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed LPS and Brefeldin A treated HUVEC cells using IL-6 antibody (66146-1-Ig, Clone: 1A3B4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

• Featured Product
• KD/KO Validated

IL-6 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 66146-1-Ig

Clone No.1A3B4

Publications(219)

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

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Tested Applications

• Positive WB detected in: U-87 MG cells, Recombinant protein, Jurkat cells, HUVEC cells
• Positive IF/ICC detected in: LPS and Brefeldin A treated HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 153 publications below
• IHC: See 55 publications below
• IF: See 32 publications below
• ELISA: See 2 publications below

Product Information

66146-1-Ig targets IL-6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, sheep
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: IL-6 fusion protein Ag10646
• Full Name: interleukin 6 (interferon, beta 2)
• Calculated Molecular Weight: 212 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC015511
• Gene Symbol: IL6
• Gene ID (NCBI): 3569
• ENSEMBL Gene ID: ENSG00000136244
• RRID: AB_2881543
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05231
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Publications

Species	Application	Title
human	WB	Cell Metab; Lighting up arginine metabolism reveals its functional diversity in physiology and pathology; Authors - Rui Li
pig	IHC	Bioact Mater; Angiogenesis, hemocompatibility and bactericidal effect of bioactive natural polymer-based bilayer adhesive skin substitute for infected burned wound healing; Authors - Mina Shahriari-Khalaji
human	WB	Nat Commun; A stress-induced cilium-to-PML-NB route drives senescence initiation; Authors - Xiaoyu Ma
	WB	Acta Pharm Sin B; Ligand-based substituent-anchoring design of selective receptor-interacting protein kinase 1 necroptosis inhibitors for ulcerative colitis therapy; Authors - Jing Zhu
	IF	Nat Commun; Catalytically potent and selective clusterzymes for modulation of neuroinflammation through single-atom substitutions.; Authors - Haile Liu
	WB	Acta Biomater; Porous composite hydrogels with improved MSC survival for robust epithelial sealing around implants and M2 macrophage polarization; Authors - Yongzheng Li