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IL-6 Monoclonal antibody

IL-6 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A3B4

Cat no : 66146-1-Ig

Synonyms

B cell stimulatory factor 2, BSF 2, BSF2, CDF, CTL differentiation factor, HSF, Hybridoma growth factor, IL 6, IL6, IL-6, Interferon beta 2, Interleukin 6



Tested Applications

Positive WB detected inU-87 MG cells, Recombinant protein, Jurkat cells, HUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 66146-1-Ig is IL-6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IL-6 fusion protein Ag10646
Full Name interleukin 6 (interferon, beta 2)
Calculated molecular weight 212 aa, 24 kDa
Observed molecular weight24 kDa
GenBank accession numberBC015511
Gene symbol IL6
Gene ID (NCBI) 3569
RRIDAB_2881543
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Protocols

Product Specific Protocols
WB protocol for IL-6 antibody 66146-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIHC

Bioact Mater

Angiogenesis, hemocompatibility and bactericidal effect of bioactive natural polymer-based bilayer adhesive skin substitute for infected burned wound healing

Authors - Mina Shahriari-Khalaji
humanWB

Nat Commun

A stress-induced cilium-to-PML-NB route drives senescence initiation

Authors - Xiaoyu Ma
WB

Acta Pharm Sin B

Ligand-based substituent-anchoring design of selective receptor-interacting protein kinase 1 necroptosis inhibitors for ulcerative colitis therapy

Authors - Jing Zhu
IF

Nat Commun

Catalytically potent and selective clusterzymes for modulation of neuroinflammation through single-atom substitutions.

Authors - Haile Liu
WB

Acta Biomater

Porous composite hydrogels with improved MSC survival for robust epithelial sealing around implants and M2 macrophage polarization

Authors - Yongzheng Li
IHC

Aging Dis

Sterol-resistant SCAP Overexpression in Vascular Smooth Muscle Cells Accelerates Atherosclerosis by Increasing Local Vascular Inflammation through Activation of the NLRP3 Inflammasome in Mice.

Authors - Danyang Li