IL-6 Recombinant monoclonal antibody, PBS Only (Detector)

• 

  Sandwich ELISA standard curve of MP00687-4

  Sandwich ELISA standard curve of MP00687-4, Human IL6 Recombinant Matched Antibody Pair - PBS only. 83747-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0117. 83747-9-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL

• 

  Sample test of MP00687-4

  Human peripheral blood mononuclear cells (PBMC) (1 x 10^6 cells/mL) were cultured in DMEM supplemented with 8% fetal bonive serum, 5μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days and 5 days. Aliquots of the cell culture supernates were removed and assayed for levels of human IL6.

IL-6 antibodies are used for both research and therapeutic purposes to detect, quantify, and block the activity of interleukin-6 (IL-6), a cytokine central to immune regulation, inflammation, hematopoiesis, and cancer biology. Proteintech has an extensive range of IL-6 antibodies validated for WB, IHC, IF/ICC, IP, Neutralization, ELISA and more.

Cat No. 83747-6-PBS

Clone No.240669A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

---

Product Information

83747-6-PBS targets IL-6 in Sandwich ELISA, Indirect ELISA, Sample test applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: interleukin 6 (interferon, beta 2)
• Calculated Molecular Weight: 212 aa, 24 kDa
• GenBank Accession Number: BC015511
• Gene Symbol: IL6
• Gene ID (NCBI): 3569
• ENSEMBL Gene ID: ENSG00000136244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05231
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.