CD127/IL-7R Polyclonal antibody

CD127/IL-7R Polyclonal Antibody for FC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, FC, ELISA

Conjugate

Unconjugated

Cat no : 17626-1-AP

Synonyms

CD127, CDW127, IL 7 receptor subunit alpha, IL 7R alpha, IL 7R subunit alpha, IL 7RA, IL7R, IL-7R, IL7RA, ILRA, interleukin 7 receptor



Tested Applications

Positive WB detected inmouse thymus tissue, K-562 cells
Positive IP detected inK-562 cells
Positive FC detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3600
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

17626-1-AP targets CD127/IL-7R in WB, IP, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD127/IL-7R fusion protein Ag11844
Full Name interleukin 7 receptor
Calculated molecular weight 459 aa, 52 kDa
Observed molecular weight 56-58 kDa
GenBank accession numberBC067537
Gene symbol IL7R
Gene ID (NCBI) 3575
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD127, also known as IL-7R subunit alpha (IL-7RA), is a type I membrane glycoprotein expressed on thymocytes, B cell precursors, most T cells, and some lymphoid and myeloid cells. IL-7R is a heterodimer composed of CD127 and the common-γ chain receptor (CD132). IL-7R plays critical roles in lymphocyte development and homeostasis. CD127 can also act as a receptor for thymic stromal lymphopoietin (TSLP).

Protocols

Product Specific Protocols
WB protocol for CD127/IL-7R antibody 17626-1-APDownload protocol
IP protocol for CD127/IL-7R antibody 17626-1-APDownload protocol
FC protocol for CD127/IL-7R antibody 17626-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Med Rep

TET2 is involved in DNA hydroxymethylation, cell proliferation and inflammatory response in keratinocytes.

Authors - Xinxin Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Bastien (Verified Customer) (08-19-2020)

Western blotting of protein from B cells of mice spleen. Protein extract was loaded on 8 % polyacrylamide gel and then transfert on nitrocellulose membrane. Nitrocellulose membrane was blocked with 5% BSA during 1h. Membrane was then incubated overnight at 4°C with 1/500 IL7Ra antibody (17626-1-AP). Finally, the membrane was incubated with anti-rabbit-HRP (clean blot IPdetection reagent from thermofisher)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: B cells
CD127/IL-7R Antibody Western Blot validation (1/500 dilution) in B cells (Cat no:17626-1-AP)