ILKAP Polyclonal antibody

ILKAP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16017-1-AP

Synonyms

ILKAP, PP2C DELTA

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Validation Data Gallery

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  WB analysis of human heart using 16017-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16017-1-AP (ILKAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16017-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16017-1-AP (ILKAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16017-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16017-1-AP (ILKAP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 16017-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16017-1-AP (ILKAP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 16017-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16017-1-AP (ILKAP antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 16017-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16017-1-AP (ILKAP antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human heart tissue, HepG2 cells, K-562 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16017-1-AP targets ILKAP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ILKAP fusion protein Ag8826
• Full Name: integrin-linked kinase-associated serine/threonine phosphatase 2C
• Calculated Molecular Weight: 392 aa, 43 kDa
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: BC006576
• Gene Symbol: ILKAP
• Gene ID (NCBI): 80895
• RRID: AB_2249188
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.