ILVBL Polyclonal antibody

ILVBL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11220-1-AP

Synonyms

HACL2, EC:4.1.2.-, AHAS, Acetolactate synthase-like protein, 2-hydroxyacyl-CoA lyase 2

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Validation Data Gallery

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  WB analysis using 11220-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11220-1-AP (ILVBL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human thyroid gland using 11220-1-AP

  human thyroid gland tissue were subjected to SDS PAGE followed by western blot with 11220-1-AP (ILVBL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11220-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11220-1-AP (ILVBL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11220-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11220-1-AP (ILVBL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11220-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11220-1-AP (ILVBL antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 11220-1-AP

  Immunofluorescent analysis of Hela cells, using ILVBL antibody 11220-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A549 cells, human thyroid gland tissue, HepG2 cells, HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:800-1:3200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11220-1-AP targets ILVBL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ILVBL fusion protein Ag1729
• Full Name: ilvB (bacterial acetolactate synthase)-like
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC011761
• Gene Symbol: ILVBL
• Gene ID (NCBI): 10994
• RRID: AB_2127187
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.