IMP5 Polyclonal antibody

IMP5 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12664-1-AP

Synonyms

IMP 5, IMP5, intramembrane protease 5, SPP like 2C, SPPL2c

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Validation Data Gallery

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  WB analysis of HEK-293 using 12664-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12664-1-AP (IMP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12664-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12664-1-AP (IMP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12664-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12664-1-AP (IMP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 12664-1-AP

  IP result of anti-IMP5 (IP:12664-1-AP, 4ug; Detection:12664-1-AP 1:500) with HEK-293 cells lysate 3600ug.

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  IHC staining of human liver cancer using 12664-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12664-1-AP (IMP5 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 12664-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12664-1-AP (IMP5 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 12664-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 12664-1-AP (IMP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, human liver tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12664-1-AP targets IMP5 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IMP5 fusion protein Ag3345
• Full Name: intramembrane protease 5
• Calculated Molecular Weight: 684 aa, 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC025401
• Gene Symbol: IMP5
• Gene ID (NCBI): 162540
• RRID: AB_2296163
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; MIR-NATs repress MAPT translation and aid proteostasis in neurodegeneration.; Authors - Roberto Simone