IMPAD1 Polyclonal antibody

IMPAD1 Polyclonal Antibody for WB, ELISA

Cat No. 25739-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

IMP 3, IMPA3, IMPAD1, IMPase 3, Inositol monophosphatase 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis of 3T3-L1 using 25739-1-AP
3T3-L1 cells were subjected to SDS PAGE followed by western blot with 25739-1-AP (IMPAD1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for IMPAD1 in ?

Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

3T3-L1 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25739-1-AP targets IMPAD1 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag22629 Product name: Recombinant human IMPAD1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 34-359 aa of BC067814 Sequence: GRFSLFGLGGEPGGGAAEPAAAADGGTVDLREMLAVSVLAAVRGGDEVRRVRESNVLHEKSKGKTREGAEDKMTSGDVLSNRKMFYLLKAAFPSVQINTEEHVDAADQEVILWDHKIPEDILEEVTTPKEVPAESVTVWIDPLDATQEYTEDLRKYVTTMVCVAVNGKPMLGVIHKPFSEYTAWAMVDGGSNVKARSSYNEKTPRIVVSRSHSGMVKQVALQTFGNQTTIIPAGGAGYKVLALLDVPDKSQEKADLYIHVTYIKKWDICAGNAILKALGGHMTTLSGEEISYTGSDGIEGGLLASIRMNHQALVRKLPDLEKTGHK Predict reactive species
Full Name	inositol monophosphatase domain containing 1
Observed Molecular Weight	39 kDa
GenBank Accession Number	BC067814
Gene Symbol	IMPAD1
Gene ID (NCBI)	54928
RRID	AB_2880219
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NX62
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for IMPAD1 antibody 25739-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The IMPAD1 Polyclonal antibody (Cat# 25739-1-AP) has 1 citation to date. It was used in a Western blot study published in Nature Communications (2025), investigating mammalian tRNA acetylation and its role in translation efficiency and tRNA quality control. The research field falls within RNA biology and translational regulation.