Tested Applications
| Positive WB detected in | HeLa cells, mouse spleen tissue, A431 cells, K-562 cells, Raji cells, rat spleen tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human tonsillitis tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22092-1-AP targets IMPDH1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17297 Product name: Recombinant human IMPDH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 170-235 aa of BC033622 Sequence: LSPSHTVGDVLEAKMRHGFSGIPITETGTMGSKLVGIVTSRDIDFLAEKDHTTLLSEVMTPRIELV Predict reactive species |
| Full Name | IMP (inosine monophosphate) dehydrogenase 1 |
| Calculated Molecular Weight | 563 aa, 60 kDa |
| Observed Molecular Weight | 53-60 kDa |
| GenBank Accession Number | BC033622 |
| Gene Symbol | IMPDH1 |
| Gene ID (NCBI) | 3614 |
| RRID | AB_2878992 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20839 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IMPDH1(Inosine-5'-monophosphate dehydrogenase 1) is also named as IMPD1, IMP dehydrogenase 1 and belongs to the IMPDH/GMPR family. It catalyzes the rate limiting step of de novo guanine synthesis and an important target for the development of drugs with both chemotherapeutic and immunosuppressive activity. It may also have a role in the development of malignancy and the growth progression of some tumors. This protein has some isoforms produced by alternative splicing with the molecular mass of 53-65 kDa. This antibody may has cross reaction with IMPDH2 due to their high homology.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IMPDH1 antibody 22092-1-AP | Download protocol |
| IHC protocol for IMPDH1 antibody 22092-1-AP | Download protocol |
| IP protocol for IMPDH1 antibody 22092-1-AP | Download protocol |
| WB protocol for IMPDH1 antibody 22092-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The IMPDH1 Polyclonal antibody (Cat# 22092-1-AP) has 25 citations spanning journals including Cell, Nature Communications, Molecular Cell, Cell Reports, Molecular Therapy, PLoS Pathogens, Frontiers in Immunology, BMC Cancer, Neurobiology of Aging, and Cancers. Research fields cover purine biosynthesis, cancer biology (pancreatic, glioblastoma, bladder, breast, lymphoma, cervical, esophageal), immunotherapy resistance, viral pathogenesis, neurodegeneration, aging, and retinal development.
| Species | Application | Title |
|---|---|---|
Nat Commun Targeting spermine metabolism to overcome immunotherapy resistance in pancreatic cancer | ||
Mol Cell Accumulation of succinate suppresses de novo purine synthesis through succinylation-mediated control of the mitochondrial folate cycle. | ||
Mol Cell ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis. | ||
Int J Biol Sci LncRNA UCA1 Participates in De Novo Synthesis of Guanine Nucleotides in Bladder Cancer by Recruiting TWIST1 to Increase IMPDH1/2 | ||
Mol Ther IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.
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Reviews
What customers say
One reviewer used this IMPDH2 antibody for immunofluorescence on iPSC cells at a 1:300 dilution. The staining produced a robust, perinuclear signal visible in nearly all cells at 60× magnification. The distribution was consistent across the culture and matched patterns seen in other tested cell lines, supporting the antibody's specificity. The reviewer rated the product 4 out of 5.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Alexios (Verified Customer) (09-17-2025) | Immunofluorescence staining of iPSCs with IMPDH2 antibody showed a robust signal present in nearly all cells at 60× magnification, perinuclear. The distribution appeared consistent across the culture and aligned with patterns observed in other tested cell lines, supporting the specificity of the antibody signal.
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