IMPDH1 Polyclonal antibody

• Featured Product
• KD/KO Validated

IMPDH1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 22092-1-AP

Synonyms

IMP dehydrogenase 1, IMPD, IMPD 1, IMPD1, IMPDH 1, IMPDH I, IMPDH1, LCA11, RP10, sWSS2608

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Validation Data Gallery

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  WB analysis of HeLa using 22092-1-AP

  WB result of IMPDH1 antibody (22092-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IMPDH1 transfected HeLa cells.

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  WB analysis using 22092-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22092-1-AP (IMPDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22092-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22092-1-AP (IMPDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 22092-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 22092-1-AP (IMPDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 22092-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 22092-1-AP (IMPDH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22092-1-AP

  IP result of anti-IMPDH1 (IP:22092-1-AP, 4ug; Detection:22092-1-AP 1:2000) with HeLa cells lysate 3200ug.

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  IHC staining of human ovary tumor using 22092-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 22092-1-AP (IMPDH1 Antibody) at dilution of 1:50.

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  IHC staining of human ovary tumor using 22092-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor slide using 22092-1-AP (IMPDH1 Antibody) at dilution of 1:50.

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  IHC staining of human tonsillitis using 22092-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 22092-1-AP (IMPDH1 Antibody) at dilution of 1:50.

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  IHC staining of human tonsillitis using 22092-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 22092-1-AP (IMPDH1 Antibody) at dilution of 1:50.

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  IF Staining of HeLa using 22092-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22092-1-AP (IMPDH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse spleen tissue, A431 cells, K-562 cells, Raji cells, rat spleen tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Product Information

The immunogen of 22092-1-AP is IMPDH1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IMPDH1 fusion protein Ag17297
• Full Name: IMP (inosine monophosphate) dehydrogenase 1
• Calculated Molecular Weight: 563 aa, 60 kDa
• Observed Molecular Weight: 53-60 kDa
• GenBank Accession Number: BC033622
• Gene Symbol: IMPDH1
• Gene ID (NCBI): 3614
• RRID: AB_2878992
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IMPDH1(Inosine-5'-monophosphate dehydrogenase 1) is also named as IMPD1, IMP dehydrogenase 1 and belongs to the IMPDH/GMPR family. It catalyzes the rate limiting step of de novo guanine synthesis and an important target for the development of drugs with both chemotherapeutic and immunosuppressive activity. It may also have a role in the development of malignancy and the growth progression of some tumors. This protein has some isoforms produced by alternative splicing with the molecular mass of 53-65 kDa. This antibody may has cross reaction with IMPDH2 due to their high homology.

Publications

Species	Application	Title
human	WB	Mol Cell; ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.; Authors - Eunus S Ali
human	WB,IHC,IF,IP	Mol Ther; IMPDH1/YB-1 Positive Feedback Loop Assembles Cytoophidia and Represents a Therapeutic Target in Metastatic Tumors.; Authors - Hailong Ruan ; KD Validated
human	WB	J Oncol; The Role of Purine Metabolism-Related Genes PPAT and IMPDH1 in the Carcinogenesis of Intrahepatic Cholangiocarcinoma Based on Metabonomic and Bioinformatic Analyses; Authors - Chang-Jun Liu ; KD Validated
human	WB	Cells; Distinct Classes of Flavonoids and Epigallocatechin Gallate, Polyphenol Affects an Oncogenic Mutant p53 Protein, Cell Growth and Invasion in a TNBC Breast Cancer Cell Line.; Authors - Madhu Kollareddy
human	WB	Int J Biochem Cell Biol; Characterisation of inosine monophosphate dehydrogenase expression during retinal development: differences between variants and isoforms.; Authors - Gunter Jennifer H JH
human	WB	Clin Chem Lab Med; Reference standards for the detection of anti-mitochondrial and anti-rods/rings autoantibodies.; Authors - S John Calise