IMPDH2-specific Polyclonal antibody

IMPDH2-specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55398-1-AP

Synonyms

IMP dehydrogenase 2, IMPD 2, IMPD2, IMPDH 2, IMPDH II, IMPDH2, IMPDH2-specific

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Validation Data Gallery

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  WB analysis of A549 using 55398-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 55398-1-AP (IMPDH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 55398-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55398-1-AP (IMPDH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 55398-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 55398-1-AP (IMPDH2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 55398-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55398-1-AP (IMPDH2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 55398-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 55398-1-AP (IMPDH2 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, K-562 cells, mouse skeletal muscle tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Product Information

55398-1-AP targets IMPDH2-specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: IMP (inosine monophosphate) dehydrogenase 2
• Calculated molecular weight: 56 kDa
• Observed molecular weight: 56 kDa
• GenBank accession number: NM_000884
• Gene symbol: IMPDH2
• Gene ID (NCBI): 3615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IMPDH2, also named as IMPD2, belongs to the IMPDH/GMPR family. It is a rate limiting enzyme in the de novo synthesis of guanine nucleotides and therefore is involved in the regulation of cell growth. It may also have a role in the development of malignancy and the growth progression of some tumors. IMPDH2 is a key enzyme in the purine nucleotide biosynthetic pathway and constitutes a pivotal biological target for immunosuppressant and antiviral drugs. (PMID:21181270). This antibody is specific to IMPDH2.