INADL Monoclonal antibody

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  WB analysis using 67833-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67833-1-Ig (INADL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 67833-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using INADL antibody (67833-1-Ig, Clone: 3C6A4 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

INADL Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 67833-1-Ig

Clone No.3C6A4

Publications(1)

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Synonyms

PATJ, Pals1-associated tight junction protein, InaD-like protein, hINADL, CIPP

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Tested Applications

• Positive WB detected in: LNCaP cells, HeLa cells, Jurkat cells, HEK-293 cells, K 562 cells, PC-12 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

67833-1-Ig targets INADL in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15953

  Product name: Recombinant human INADL protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 737-1079 aa of BC142661

  Sequence: LVSVNEYCLDNTSLAEAVEILKAVPPGLVHLGICKPLVEDNEEESCYILHSSSNEDKTEFSGTIHDINSSLILEAPKGFRDEPYFKEELVDEPFLDLGKSFHSQQKEIEQSKEAWEMHEFLTPRLQEMDEEREMLVDEEYELYQDPSPSMELYPLSHIQEATPVPSVNELHFGTQWLHDNEPSESQEARTGRTVYSQEAQPYGYCPENVMKENFVMESLPSVPSTEGNSQQGRFDDLENLNSLAKTSLDLGMIPNDVQGPSLLIDLPVVAQRREQEDLPLYQHQATRVISKASAYTGMLSSRYATDTCELPEREEGEGEETPNFSHWGPPRIVEIFREPNVSL

• Full Name: InaD-like (Drosophila)
• Calculated Molecular Weight: 1801 aa, 196 kDa
• Observed Molecular Weight: 196 kDa
• GenBank Accession Number: BC142661
• Gene Symbol: INADL
• Gene ID (NCBI): 10207
• RRID: AB_2918595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q8NI35
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Redox Biol; PATJ regulates cell stress responses and vascular remodeling post-stroke; Authors - Mengqi Zhang ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shveta (Verified Customer) (05-13-2025)

4% PFA fixation - 20min Blocking 5% BSA - 1hr RT Primary AB - ON 4deg Secondary - 1hr RT

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:250
• Cell Tissue Type: Epithelial cells