INCENP Polyclonal antibody

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• INCENP Polyclonal antibody (29419-1-AP)

INCENP Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 29419-1-AP Copied!

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Inner centromere protein

• 

  WB analysis using 29419-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29419-1-AP (INCENP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 29419-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29419-1-AP (INCENP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 29419-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using INCENP antibody (29419-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 29419-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using INCENP antibody (29419-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 29419-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INCENP antibody (29419-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, MOLT-4 cells, Raji cells
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29419-1-AP targets INCENP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30184

  Product name: Recombinant human incenp protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 533-789 aa of NM_001040694

  Sequence: SFVEKERQRLENLRRKEEAEQLRRQKVEEDKRRRLEEVKLKREERLRKVLQARERVEQMKEEKKKQIEQKFAQIDEKTEKAKEERLAEEKAKKKAAAKKMEEVEARRKQEEEARRLRWLQQEEEERRHQELLQKKKEEEQERLRKAAEAKRLAEQREQERREQERREQERREQERREQERREQERQLAEQERRREQERLQAERELQEREKALRLQKEQLQRELEEKKKKEEQQRLAERQLQEEQEKKAKEAAGASKA

• Full Name: inner centromere protein antigens 135/155kDa
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 106-130 kDa
• GenBank Accession Number: NM_001040694
• Gene Symbol: INCENP
• Gene ID (NCBI): 3619
• RRID: AB_2918303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NQS7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The INCENP Polyclonal antibody (Cat# 29419-1-AP) has 5 total citations published in Molecular Cell, Developmental Cell, EMBO Journal, International Immunopharmacology, and Scientific Reports. Research fields span RNA m6A-mediated translation and tumor chemoresistance, chromatin regulation in neurodevelopment, centromeric chromatin integrity and chromosome segregation, gastric cancer immunotherapy, and genome maintenance via the chromosomal passenger complex. All citations used human samples with WB and/or IF applications.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance; Authors - Tao Liu
human	WB,IF	Dev Cell; The anaphase-promoting complex controls a ubiquitination-phosphoprotein axis in chromatin during neurodevelopment; Authors - Leya Ledvin
human	WB,IF	EMBO J; Nucleosome interaction of the CPC secures centromeric chromatin integrity and chromosome segregation fidelity.; Authors - Anjitha Gireesh
human	WB,IF	Int Immunopharmacol; Identification of TAP1 as a T-cell related therapeutic target in gastric cancer by mediating oxalipliatin-related synergistic enhancement of immunotherapy; Authors - Yupeng Zhao
human	WB	Sci Rep; CCDC69 maintains genome integrity by regulating KIF2C/MCAK depolymerase activity and the stability of the chromosomal passenger complex; Authors - Didier Fesquet