INCENP Polyclonal antibody

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  WB analysis using 29419-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29419-1-AP (INCENP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29419-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29419-1-AP (INCENP antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 29419-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INCENP antibody (29419-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 29419-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using INCENP antibody (29419-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HepG2 using 29419-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using INCENP antibody (29419-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

INCENP Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 29419-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Inner centromere protein

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells, MOLT-4 cells, Raji cells
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IF: See 2 publications below

Product Information

29419-1-AP targets INCENP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30184

  Product name: Recombinant human incenp protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 533-789 aa of NM_001040694

  Sequence: SFVEKERQRLENLRRKEEAEQLRRQKVEEDKRRRLEEVKLKREERLRKVLQARERVEQMKEEKKKQIEQKFAQIDEKTEKAKEERLAEEKAKKKAAAKKMEEVEARRKQEEEARRLRWLQQEEEERRHQELLQKKKEEEQERLRKAAEAKRLAEQREQERREQERREQERREQERREQERREQERQLAEQERRREQERLQAERELQEREKALRLQKEQLQRELEEKKKKEEQQRLAERQLQEEQEKKAKEAAGASKA

• Full Name: inner centromere protein antigens 135/155kDa
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 106-130 kDa
• GenBank Accession Number: NM_001040694
• Gene Symbol: INCENP
• Gene ID (NCBI): 3619
• RRID: AB_2918303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NQS7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Dev Cell; The anaphase-promoting complex controls a ubiquitination-phosphoprotein axis in chromatin during neurodevelopment; Authors - Leya Ledvin
human	WB,IF	Int Immunopharmacol; Identification of TAP1 as a T-cell related therapeutic target in gastric cancer by mediating oxalipliatin-related synergistic enhancement of immunotherapy; Authors - Yupeng Zhao
human	WB	Sci Rep; CCDC69 maintains genome integrity by regulating KIF2C/MCAK depolymerase activity and the stability of the chromosomal passenger complex; Authors - Didier Fesquet
human	WB	Mol Cell; The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance; Authors - Tao Liu