ING2 Polyclonal antibody

• Featured Product
• KD/KO Validated

ING2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 11560-1-AP

Synonyms

p33ING2, p32, Inhibitor of growth protein 2, Inhibitor of growth 1-like protein, ING1Lp

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Validation Data Gallery

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  WB analysis of HEK-293 using 11560-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11560-1-AP (ING2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11560-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11560-1-AP (ING2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11560-1-AP

  IP result of anti-ING2 (IP:11560-1-AP, 3ug; Detection:11560-1-AP 1:500) with HEK-293 cells lysate 1000ug.

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  IHC staining of human colon cancer using 11560-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 11560-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human cervical cancer using 11560-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 11560-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 11560-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 11560-1-AP (ING2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human liver tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

11560-1-AP targets ING2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ING2 fusion protein Ag2121
• Full Name: inhibitor of growth family, member 2
• Calculated Molecular Weight: 280 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC030128
• Gene Symbol: ING2
• Gene ID (NCBI): 3622
• RRID: AB_2127771
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ING2, also named as ING1L, belongs to the ING family and contains a PHD-type zinc finger. It is induced by the DNA-damaging agent neocarzinostatin. As a candidate tumor suppressor, ING2 seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. It is a component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. Recent finding reported that ING2 regulates muscle differentiation by regulating chormatin remodeling program. Catalog#11560-1-AP is a rabbit polyclonal antibody raised against full length ING2 of human origin.

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Human family with sequence similarity 60 member A (FAM60A) protein: a new subunit of the Sin3 deacetylase complex.; Authors - Smith Karen T KT
human	WB	Chem Biol; Deacetylase inhibitors dissociate the histone-targeting ING2 subunit from the Sin3 complex.; Authors - Smith Karen T KT ; KD Validated
human	WB	Cancers (Basel); Antithetic hTERT Regulation by Androgens in Prostate Cancer Cells: hTERT Inhibition Is Mediated by the ING1 and ING2 Tumor Suppressors.; Authors - Sophie Bartsch ; KD Validated
human,mouse	WB,IHC,IF	Front Cell Dev Biol; ING2 Controls Mitochondrial Respiration via Modulating MRPL12 Ubiquitination in Renal Tubular Epithelial Cells.; Authors - Ying Yang ; KD Validated
human	IHC	Cancer Lett; Decreased expression of ING2 gene and its clinicopathological significance in hepatocellular carcinoma.; Authors - Zhang Hua-kun HK
	IP	Biochem J; Histone H3 N-terminal mimicry drives a novel network of methyl-effector interactions.; Authors - Jianji Chen