Tested Applications
| Positive WB detected in | HEK-293 cells, human liver tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human colon cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11560-1-AP targets ING2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2121 Product name: Recombinant human ING2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-280 aa of BC030128 Sequence: MLGQQQQQLYSSAALLTGERSRLLTCYVQDYLECVESLPHDMQRNVSVLRELDNKYQETLKEIDDVYEKYKKEDDLNQKKRLQQLLQRALINSQELGDEKIQIVTQMLELVENRARQMELHSQCFQDPAESERASDKAKMDSSQPERSSRRPRRQRTSESRDLCHMANGIEDCDDQPPKEKKSKSAKKKKRSKAKQEREASPVEFAIDPNEPTYCLCNQVSYGEMIGCDNEQCPIEWFHFSCVSLTYKPKGKWYCPKCRGDNEKTMDKSTEKTKKDRRSR Predict reactive species |
| Full Name | inhibitor of growth family, member 2 |
| Calculated Molecular Weight | 280 aa, 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC030128 |
| Gene Symbol | ING2 |
| Gene ID (NCBI) | 3622 |
| RRID | AB_2127771 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H160 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ING2, also named as ING1L, belongs to the ING family and contains a PHD-type zinc finger. It is induced by the DNA-damaging agent neocarzinostatin. As a candidate tumor suppressor, ING2 seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. It is a component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. Recent finding reported that ING2 regulates muscle differentiation by regulating chormatin remodeling program. Catalog#11560-1-AP is a rabbit polyclonal antibody raised against full length ING2 of human origin.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ING2 antibody 11560-1-AP | Download protocol |
| IHC protocol for ING2 antibody 11560-1-AP | Download protocol |
| IP protocol for ING2 antibody 11560-1-AP | Download protocol |
| WB protocol for ING2 antibody 11560-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ING2 Polyclonal antibody (Cat# 11560-1-AP) has 12 citations published in journals including Cancer Letters, Molecular & Cellular Proteomics, Chemistry & Biology, Frontiers in Cell and Developmental Biology, Molecular Medicine Reports, Journal of Molecular Medicine, Cancers, Biochemical Journal, Digestive Diseases and Sciences, PLoS One, and Oncology Letters. Research fields span hepatocellular carcinoma, gastric cancer, prostate cancer, mitochondrial respiration, chromatin remodeling, androgen receptor signaling, and muscle differentiation.
| Species | Application | Title |
|---|---|---|
Cancer Lett Decreased expression of ING2 gene and its clinicopathological significance in hepatocellular carcinoma. | ||
Mol Cell Proteomics Human family with sequence similarity 60 member A (FAM60A) protein: a new subunit of the Sin3 deacetylase complex. | ||
Chem Biol Deacetylase inhibitors dissociate the histone-targeting ING2 subunit from the Sin3 complex.
| ||
Front Cell Dev Biol ING2 Controls Mitochondrial Respiration via Modulating MRPL12 Ubiquitination in Renal Tubular Epithelial Cells.
| ||
J Mol Med (Berl) A novel crosstalk between the tumor suppressors ING1 and ING2 regulates androgen receptor signaling. | ||
Mol Med Rep Expression profiles of inhibitor of growth protein 2 in normal and cancer tissues: An immunohistochemical screening analysis. |
Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The reviewer used the antibody at a 1:5000 dilution for Western blot on HEK293T cells and reported reliable performance, noting clear and specific bands with minimal background. They also highlighted consistent results across replicates, indicating the antibody performs well for detecting ING2 by WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Rahul (Verified Customer) (07-23-2025) | Reliable for Western blot. Clear, specific bands with minimal background. ING2 Ab. performs consistently across replicates.
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