CoraLite® Plus 488-conjugated ING2 Monoclonal antibody

ING2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C3A7

Cat no : CL488-66512

Synonyms

ING1L, ING1Lp, ING2, Inhibitor of growth protein 2, p32, p33ING2

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Validation Data Gallery

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  FC experiment of HepG2 using CL488-66512

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human ING2 (CL488-66512, Clone:2C3A7) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66512 targets ING2 in FC (Intra) applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ING2 fusion protein Ag19291
• Full Name: inhibitor of growth family, member 2
• Calculated Molecular Weight: 280 aa, 33 kDa
• Observed Molecular Weight: 33-35 kDa
• GenBank Accession Number: BC030128
• Gene Symbol: ING2
• Gene ID (NCBI): 3622
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ING2, also named as ING1L, belongs to the ING family and contains a PHD-type zinc finger. As a candidate tumor suppressor, ING2 seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. It is a component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. Recent finding reported that ING2 regulates muscle differentiation by regulating chormatin remodeling program.