ING4 Monoclonal antibody

ING4 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1


Human, pig, rabbit, rat







Cat no : 67754-1-Ig


ING4, Inhibitor of growth protein 4, my036, p29ING4

Tested Applications

Positive WB detected inHEK-293 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, HeLa cells, Jurkat cells, MOLT-4 cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman colon cancer tissue

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67754-1-Ig targets ING4 in WB, IHC, IF, ELISA applications and shows reactivity with Human, pig, rabbit, rat samples.

Tested Reactivity Human, pig, rabbit, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ING4 fusion protein Ag4610
Full Name inhibitor of growth family, member 4
Calculated Molecular Weight 29 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC007781
Gene Symbol ING4
Gene ID (NCBI) 51147
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ING4, also named as p29ING4, belongs to the ING family. It is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. It may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. ING4 can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. It may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1). ING4 is a tumor suppressor gene that interacts with NFkB and represses its transcriptional activity. Several lines of evidence suggest that the tumor suppressor gene ING4, NFkB and its target genes matrix metalloproteases MMP-2, MMP-9 and u-PA are critically involved in tumor invasion.


Product Specific Protocols
WB protocol for ING4 antibody 67754-1-IgDownload protocol
IHC protocol for ING4 antibody 67754-1-IgDownload protocol
IF protocol for ING4 antibody 67754-1-IgDownload protocol
Standard Protocols
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