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Inhibin Beta A Polyclonal antibody
Inhibin Beta A Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 10651-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, MCF-7 cells, A549 cells, SGC-7901 cells, BGC-823 cells, HepG2 cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 4 publications below |
IHC | See 10 publications below |
IF | See 2 publications below |
Product Information
10651-1-AP targets Inhibin Beta A in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse, Hu Sheep, sheep |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Inhibin Beta A fusion protein Ag1001 |
Full Name | inhibin, beta A |
Calculated molecular weight | 47 kDa |
Observed molecular weight | 47 kDa |
GenBank accession number | BC007858 |
Gene symbol | INHBA |
Gene ID (NCBI) | 3624 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Inhibin is a 32-kD dimeric glycoprotein consisting of an alpha subunit and one of two beta subunits (beta A or beta B) leading to inhibin A or B, respectively, which was isolated and cloned on the basis of its ability to inhibit FSH release from the pituitary. Inhibin beta A forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin beta A can also bind the beta B and A isoforms to form activin AB and inhibin, respectively.
Protocols
Product Specific Protocols | |
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WB protocol for Inhibin Beta A antibody 10651-1-AP | Download protocol |
IHC protocol for Inhibin Beta A antibody 10651-1-AP | Download protocol |
IF protocol for Inhibin Beta A antibody 10651-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation. | ||
Development Combinatorial actions of Tgfβ and Activin ligands promote oligodendrocyte development and CNS myelination. | ||
PLoS One Action mechanism of inhibin α-subunit on the development of Sertoli cells and first wave of spermatogenesis in mice. | ||
Transl Oncol Multiregion single cell analysis reveals a novel subtype of cancer-associated fibroblasts located in the hypoxic tumor microenvironment in colorectal cancer | ||
BMC Cancer Esophageal squamous cell carcinoma invasion is inhibited by Activin A in ACVRIB-positive cells. |