Validation Data Gallery
|Positive WB detected in||HeLa cells, A549 cells, BGC-823 cells, SGC-7901 cells, MCF-7 cells, HepG2 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A549 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10651-1-AP targets Inhibin Beta A in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, Hu Sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Inhibin Beta A fusion protein Ag1001|
|Full Name||inhibin, beta A|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC007858|
|Gene ID (NCBI)||3624|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Inhibin is a 32-kD dimeric glycoprotein consisting of an alpha subunit and one of two beta subunits (beta A or beta B) leading to inhibin A or B, respectively, which was isolated and cloned on the basis of its ability to inhibit FSH release from the pituitary. Inhibin beta A forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin beta A can also bind the beta B and A isoforms to form activin AB and inhibin, respectively.
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