• Featured Product
  • KD/KO Validated

Inhibin Beta A Polyclonal antibody

Inhibin Beta A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10651-1-AP

Synonyms

Activin beta A chain, EDF, FRP, INHBA, Inhibin Beta A, Inhibin beta A chain, Inhibin β-A, inhibin, beta A



Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, A549 cells, SGC-7901 cells, BGC-823 cells, HepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10651-1-AP targets Inhibin Beta A in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, Hu Sheep, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Inhibin Beta A fusion protein Ag1001
Full Name inhibin, beta A
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC007858
Gene symbol INHBA
Gene ID (NCBI) 3624
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inhibin is a 32-kD dimeric glycoprotein consisting of an alpha subunit and one of two beta subunits (beta A or beta B) leading to inhibin A or B, respectively, which was isolated and cloned on the basis of its ability to inhibit FSH release from the pituitary. Inhibin beta A forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin beta A can also bind the beta B and A isoforms to form activin AB and inhibin, respectively.

Protocols

Product Specific Protocols
WB protocol for Inhibin Beta A antibody 10651-1-APDownload protocol
IHC protocol for Inhibin Beta A antibody 10651-1-APDownload protocol
IF protocol for Inhibin Beta A antibody 10651-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.

Authors - Yi-Ing Chen

Development

Combinatorial actions of Tgfβ and Activin ligands promote oligodendrocyte development and CNS myelination.

Authors - Dipankar J Dutta
mouseWB

PLoS One

Action mechanism of inhibin α-subunit on the development of Sertoli cells and first wave of spermatogenesis in mice.

Authors - Cai Kailai K
humanIHC,IF

Transl Oncol

Multiregion single cell analysis reveals a novel subtype of cancer-associated fibroblasts located in the hypoxic tumor microenvironment in colorectal cancer

Authors - Nanxin Zheng

Hepatol Commun

Activin A is a prominent autocrine regulator of hepatocyte growth arrest.

Authors - Srividyameena Haridoss

BMC Cancer

Esophageal squamous cell carcinoma invasion is inhibited by Activin A in ACVRIB-positive cells.

Authors - Holli A Loomans