Inhibin Beta A Polyclonal antibody

• Featured Product
• KD/KO Validated

Inhibin Beta A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 10651-1-AP

Synonyms

Activin beta A chain, EDF, FRP, INHBA, Inhibin Beta A, Inhibin beta A chain, Inhibin β-A, inhibin, beta A

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Validation Data Gallery

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  WB analysis using 10651-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BGC-823 using 10651-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10651-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10651-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 10651-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SGC-7901 using 10651-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10651-1-AP (Inhibin beta A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10651-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10651-1-AP (Inhibin beta A antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 10651-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10651-1-AP (Inhibin beta A antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A549 using 10651-1-AP

  Immunofluorescent analysis of A549 cells using 10651-1-AP (Inhibin beta A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, A549 cells, SGC-7901 cells, BGC-823 cells, HepG2 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 10 publications below
• IF: See 2 publications below

Product Information

10651-1-AP targets Inhibin Beta A in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, Hu Sheep, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Inhibin Beta A fusion protein Ag1001
• Full Name: inhibin, beta A
• Calculated molecular weight: 47 kDa
• Observed molecular weight: 47 kDa
• GenBank accession number: BC007858
• Gene symbol: INHBA
• Gene ID (NCBI): 3624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inhibin is a 32-kD dimeric glycoprotein consisting of an alpha subunit and one of two beta subunits (beta A or beta B) leading to inhibin A or B, respectively, which was isolated and cloned on the basis of its ability to inhibit FSH release from the pituitary. Inhibin beta A forms a disulfide-linked homo-dimer known as activin A, which has been implicated in multiple biologic processes, including neoplastic progression. The inhibin beta A can also bind the beta B and A isoforms to form activin AB and inhibin, respectively.

Publications

Species	Application	Title
human	IF	Nat Commun; Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.; Authors - Yi-Ing Chen
		Development; Combinatorial actions of Tgfβ and Activin ligands promote oligodendrocyte development and CNS myelination.; Authors - Dipankar J Dutta
mouse	WB	PLoS One; Action mechanism of inhibin α-subunit on the development of Sertoli cells and first wave of spermatogenesis in mice.; Authors - Cai Kailai K
human	IHC,IF	Transl Oncol; Multiregion single cell analysis reveals a novel subtype of cancer-associated fibroblasts located in the hypoxic tumor microenvironment in colorectal cancer; Authors - Nanxin Zheng
		Hepatol Commun; Activin A is a prominent autocrine regulator of hepatocyte growth arrest.; Authors - Srividyameena Haridoss
		BMC Cancer; Esophageal squamous cell carcinoma invasion is inhibited by Activin A in ACVRIB-positive cells.; Authors - Holli A Loomans

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