|Positive WB detected in||HeLa cells, HepG2 cells, Jurkat cells, HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Sample-dependent, check data in validation data gallery|
18810-1-AP targets INO80 in WB, IP, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||INO80 homolog (S. cerevisiae)|
|Calculated molecular weight||1556 aa, 177 kDa|
|Observed molecular weight||178 kDa|
|GenBank accession number||BC146785|
|Gene ID (NCBI)||54617|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
INO80, also named as INOC1 and KIAA1259, belongs to the SNF2/RAD54 helicase family. The INO80 complex remodels chromatin by shifting nucleosomes. INO80 recruited by YY1 to YY1-activated INO80. In vitro, INO80 has double stranded DNA-dependent ATPase activity
Mammalian SWI/SNF continuously restores local accessibility to chromatin.
Cell Stem Cell
INO80 Facilitates Pluripotency Gene Activation in Embryonic Stem Cell Self-Renewal, Reprogramming, and Blastocyst Development.
Nascent RNA antagonizes the interaction of a set of regulatory proteins with chromatin.
Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.
Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair
Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
G (Verified Customer) (06-13-2022)
INO80 worked well at 1:5000 in our western blots of nuclear extracts from rodent pancreatic beta cells. 1-2ug of antibody was enough for IP reactions. Seems to work in IHC-paraffin of rodent pancreatic tissue although we are currently optimizing conditions.