• Featured Product
  • KD/KO Validated

INO80 Polyclonal antibody

INO80 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 18810-1-AP

Synonyms

INO 80, hINO80, EC:3.6.4.-, DNA helicase-related protein INO80, DNA helicase-related INO80 complex homolog 1



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18810-1-AP targets INO80 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name INO80 homolog (S. cerevisiae)
Calculated Molecular Weight 1556 aa, 177 kDa
Observed Molecular Weight 178 kDa
GenBank Accession NumberBC146785
Gene Symbol INO80
Gene ID (NCBI) 54617
RRIDAB_10598463
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INO80, also named as INOC1 and KIAA1259, belongs to the SNF2/RAD54 helicase family. The INO80 complex remodels chromatin by shifting nucleosomes. INO80 recruited by YY1 to YY1-activated INO80. In vitro, INO80 has double stranded DNA-dependent ATPase activity

Protocols

Product Specific Protocols
WB protocol for INO80 antibody 18810-1-APDownload protocol
IF protocol for INO80 antibody 18810-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Genet

Mammalian SWI/SNF continuously restores local accessibility to chromatin.

Authors - Mario Iurlaro
  • KO Validated
mouseWB,IF,ChIP

Cell Stem Cell

INO80 Facilitates Pluripotency Gene Activation in Embryonic Stem Cell Self-Renewal, Reprogramming, and Blastocyst Development.

Authors - Li Wang
  • KD Validated
mouseWB

Mol Cell

Nascent RNA antagonizes the interaction of a set of regulatory proteins with chromatin.

Authors - Lenka Skalska
mousechIP

Mol Cell

Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.

Authors - Yong Xue
  • KD Validated
humanWB

Sci Adv

Deficiency in mammalian STN1 promotes colon cancer development via inhibiting DNA repair

Authors - Dinh Duc Nguyen
humanWB,IF

Nat Commun

Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability.

Authors - Lisa Prendergast
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

G (Verified Customer) (06-13-2022)

INO80 worked well at 1:5000 in our western blots of nuclear extracts from rodent pancreatic beta cells. 1-2ug of antibody was enough for IP reactions. Seems to work in IHC-paraffin of rodent pancreatic tissue although we are currently optimizing conditions.

  • Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Mouse pancreatic tissue