|Positive WB detected in||HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18810-1-AP targets INO80 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||INO80 homolog (S. cerevisiae)|
|Calculated molecular weight||1556 aa, 177 kDa|
|Observed molecular weight||178 kDa|
|GenBank accession number||BC146785|
|Gene ID (NCBI)||54617|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
INO80, also named as INOC1 and KIAA1259, belongs to the SNF2/RAD54 helicase family. The INO80 complex remodels chromatin by shifting nucleosomes. INO80 recruited by YY1 to YY1-activated INO80. In vitro, INO80 has double stranded DNA-dependent ATPase activity
Life Sci Alliance
BZLF1 interacts with chromatin remodelers promoting escape from latent infections with EBV.
LINC00839 promotes colorectal cancer progression by recruiting RUVBL1/Tip60 complexes to activate NRF1.
The Mouse INO80 Chromatin Remodeling Complex Is an Essential Meiotic Factor for Spermatogenesis.
J Cell Mol Med
INO80 participates in the pathogenesis of recurrent miscarriage by epigenetically regulating trophoblast migration and invasion.
Int J Biol Sci
Photobiomodulation Drives MiR-136-5p Expression to Promote Injury Repair after Myocardial Infarction.
Ino80 promotes cervical cancer tumorigenesis by activating Nanog expression.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
G (Verified Customer) (06-13-2022)
INO80 worked well at 1:5000 in our western blots of nuclear extracts from rodent pancreatic beta cells. 1-2ug of antibody was enough for IP reactions. Seems to work in IHC-paraffin of rodent pancreatic tissue although we are currently optimizing conditions.