• Featured Product
  • KD/KO Validated

INO80 Polyclonal antibody

INO80 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 18810-1-AP

Synonyms

hINO80, INO80, INO80 homolog (S. cerevisiae), INO80A, INOC1, KIAA1259



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18810-1-AP targets INO80 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name INO80 homolog (S. cerevisiae)
Calculated molecular weight 1556 aa, 177 kDa
Observed molecular weight 178 kDa
GenBank accession numberBC146785
Gene symbol INO80
Gene ID (NCBI) 54617
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

INO80, also named as INOC1 and KIAA1259, belongs to the SNF2/RAD54 helicase family. The INO80 complex remodels chromatin by shifting nucleosomes. INO80 recruited by YY1 to YY1-activated INO80. In vitro, INO80 has double stranded DNA-dependent ATPase activity

Protocols

Product Specific Protocols
WB protocol for INO80 antibody 18810-1-APDownload protocol
IHC protocol for INO80 antibody 18810-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Life Sci Alliance

BZLF1 interacts with chromatin remodelers promoting escape from latent infections with EBV.

Authors - Marisa Schaeffner
  • KD Validated
humanWB

EMBO Rep

LINC00839 promotes colorectal cancer progression by recruiting RUVBL1/Tip60 complexes to activate NRF1.

Authors - Xiaoting Liu
IF

Biol Reprod

The Mouse INO80 Chromatin Remodeling Complex Is an Essential Meiotic Factor for Spermatogenesis.

Authors - Daniel W Serber
  • KO Validated
humanWB, IF

J Cell Mol Med

INO80 participates in the pathogenesis of recurrent miscarriage by epigenetically regulating trophoblast migration and invasion.

Authors - Shu Xian
mouseWB

Int J Biol Sci

Photobiomodulation Drives MiR-136-5p Expression to Promote Injury Repair after Myocardial Infarction.

Authors - Xinlu Gao
humanWB

Oncotarget

Ino80 promotes cervical cancer tumorigenesis by activating Nanog expression.

Authors - Jing Hu
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

G (Verified Customer) (06-13-2022)

INO80 worked well at 1:5000 in our western blots of nuclear extracts from rodent pancreatic beta cells. 1-2ug of antibody was enough for IP reactions. Seems to work in IHC-paraffin of rodent pancreatic tissue although we are currently optimizing conditions.

  • Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Mouse pancreatic tissue