Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, HEK-293 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18810-1-AP targets INO80 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | INO80 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 1556 aa, 177 kDa |
| Observed Molecular Weight | 178 kDa |
| GenBank Accession Number | BC146785 |
| Gene Symbol | INO80 |
| Gene ID (NCBI) | 54617 |
| RRID | AB_10598463 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULG1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
INO80, also named as INOC1 and KIAA1259, belongs to the SNF2/RAD54 helicase family. The INO80 complex remodels chromatin by shifting nucleosomes. INO80 recruited by YY1 to YY1-activated INO80. In vitro, INO80 has double stranded DNA-dependent ATPase activity
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for INO80 antibody 18810-1-AP | Download protocol |
| WB protocol for INO80 antibody 18810-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The INO80 antibody (Cat# 18810-1-AP) has 24 citations spanning journals including Cell, Nature Genetics, Cell Stem Cell, Nature Communications, Molecular Cell, Nucleic Acids Research, Science Advances, Oncogene, EMBO Journal, Genes & Development, eLife, and others. Research fields cover chromatin remodeling, transcription regulation, cancer (lung, melanoma, cervical, colorectal, endometrial, breast), stem cell biology, development, DNA repair, epigenetics, spermatogenesis, viral infection, and osteoporosis.
| Species | Application | Title |
|---|---|---|
Nat Genet Mammalian SWI/SNF continuously restores local accessibility to chromatin.
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Cell Stem Cell INO80 Facilitates Pluripotency Gene Activation in Embryonic Stem Cell Self-Renewal, Reprogramming, and Blastocyst Development.
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Nat Commun The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors | ||
Nat Commun Resolution of R-loops by INO80 promotes DNA replication and maintains cancer cell proliferation and viability.
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Mol Cell Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.
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Reviews
What customers say
The single review rates the antibody 4 out of 5 stars. The user tested it on mouse pancreatic tissue for Western Blot, Immunoprecipitation, and Immunohistochemistry. At a 1:5000 dilution, it performed well in Western blots of nuclear extracts from rodent pancreatic beta cells. For IP reactions, 1–2 µg of antibody was sufficient. The reviewer also noted it appeared to work in IHC-paraffin on rodent pancreatic tissue, though they were still optimizing the conditions at the time of writing.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
G (Verified Customer) (06-13-2022) | INO80 worked well at 1:5000 in our western blots of nuclear extracts from rodent pancreatic beta cells. 1-2ug of antibody was enough for IP reactions. Seems to work in IHC-paraffin of rodent pancreatic tissue although we are currently optimizing conditions.
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