INO80 Polyclonal antibody

• Featured Product
• KD/KO Validated

INO80 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC

Conjugate

Unconjugated

Publications

4

Cat no : 24819-1-AP

Synonyms

hINO80, INO80, INO80 homolog (S. cerevisiae), INO80A, INOC1, KIAA1259

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Validation Data Gallery

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  WB analysis of HeLa using 24819-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24819-1-AP (INO80 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24819-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24819-1-AP (INO80 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24819-1-AP

  IP Result of anti-INO80 (IP:24819-1-AP, 4ug; Detection:24819-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human colon cancer using 24819-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24819-1-AP (INO80 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24819-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24819-1-AP (INO80 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below

Product Information

The immunogen of 24819-1-AP is INO80 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INO80 fusion protein Ag20309
• Full Name: INO80 homolog (S. cerevisiae)
• Calculated molecular weight: 1556 aa, 177 kDa
• Observed molecular weight: 177 kDa, 150 kDa
• GenBank accession number: BC146785
• Gene symbol: INO80
• Gene ID (NCBI): 54617
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.

Publications

Species	Application	Title
human	WB	J Allergy Clin Immunol; An inherited immunoglobulin class-switch recombination deficiency associated with a defect in the INO80 chromatin remodeling complex.; Authors - Sven Kracker
		Genes Dev; INO80 governs superenhancer-mediated oncogenic transcription and tumor growth in melanoma.; Authors - Bingying Zhou
human	WB	Sci Rep; Beyond autophagy: a novel role for autism-linked Wdfy3 in brain mitophagy.; Authors - Eleonora Napoli ; KD Validated
human	WB	FEBS J; The chromatin remodeling protein INO80 contributes to the removal of H2A.Z at the p53-binding site of the p21 gene in response to doxorubicin.; Authors - Jian Ding ; KD Validated