INO80 Polyclonal antibody

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  WB analysis of HeLa using 24819-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24819-1-AP (INO80 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24819-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24819-1-AP (INO80 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24819-1-AP

  IP result of anti-INO80 (IP:24819-1-AP, 4ug; Detection:24819-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human colon cancer using 24819-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24819-1-AP (INO80 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 24819-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24819-1-AP (INO80 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

INO80 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 24819-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ChIP, ELISA

Synonyms

hINO80, INO80, INO80 homolog (S. cerevisiae), INO80A, INOC1, KIAA1259

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• ChIP: See 1 publications below

Product Information

24819-1-AP targets INO80 in WB, IP, IHC, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20309

  Product name: Recombinant human INO80 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1217-1556 aa of BC146785

  Sequence: KQVTVYRLICKGTIEERILQRAKEKSEIQRMVISGGNFKPDTLKPKEVVSLLLDDEELEKKLRLRQEEKRQQEETNRVKERKRKREKYAEKKKKEDELDGKRRKEGVNLVIPFVPSADNSNLSADGDDSFISVDSAMPSPFSEISISSELHTGSIPLDESSSDMLVIVDDPASSAPQSRATNSPASITGSVSDTVNGISIQEMPAAGRGHSARSRGRPKGSGSTAKGAGKGRSRKSTAGSAAAMAGAKAGAAAASAAAYAAYGYNVSKGISASSPLQTSLVRPAGLADFGPSSASSPLSSPLSKGNNVPGNPKNLHMTSSLAPDSLVRKQGKGTNPSGGR

• Full Name: INO80 homolog (S. cerevisiae)
• Calculated Molecular Weight: 1556 aa, 177 kDa
• Observed Molecular Weight: 177 kDa, 150 kDa
• GenBank Accession Number: BC146785
• Gene Symbol: INO80
• Gene ID (NCBI): 54617
• RRID: AB_2879741
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9ULG1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Allergy Clin Immunol; An inherited immunoglobulin class-switch recombination deficiency associated with a defect in the INO80 chromatin remodeling complex.; Authors - Sven Kracker
		Genes Dev; INO80 governs superenhancer-mediated oncogenic transcription and tumor growth in melanoma.; Authors - Bingying Zhou
human	WB,ChIP	Int J Mol Sci; Feedback Modulation between Human INO80 Chromatin Remodeling Complex and miR-372 in HCT116 Cells; Authors - Junaid Ali Shah ; KD Validated
human	WB	Sci Rep; Beyond autophagy: a novel role for autism-linked Wdfy3 in brain mitophagy.; Authors - Eleonora Napoli ; KD Validated
human	WB	FEBS J; The chromatin remodeling protein INO80 contributes to the removal of H2A.Z at the p53-binding site of the p21 gene in response to doxorubicin.; Authors - Jian Ding ; KD Validated