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INO80 Polyclonal antibody
INO80 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IP, IHC
Conjugate
Unconjugated
Cat no : 24819-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, HepG2 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 3 publications below |
Product Information
The immunogen of 24819-1-AP is INO80 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | INO80 fusion protein Ag20309 |
Full Name | INO80 homolog (S. cerevisiae) |
Calculated molecular weight | 1556 aa, 177 kDa |
Observed molecular weight | 177 kDa, 150 kDa |
GenBank accession number | BC146785 |
Gene symbol | INO80 |
Gene ID (NCBI) | 54617 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.
Protocols
Product Specific Protocols | |
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WB protocol for INO80 antibody 24819-1-AP | Download protocol |
IHC protocol for INO80 antibody 24819-1-AP | Download protocol |
IP protocol for INO80 antibody 24819-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Allergy Clin Immunol An inherited immunoglobulin class-switch recombination deficiency associated with a defect in the INO80 chromatin remodeling complex. | ||
Genes Dev INO80 governs superenhancer-mediated oncogenic transcription and tumor growth in melanoma. | ||
Sci Rep Beyond autophagy: a novel role for autism-linked Wdfy3 in brain mitophagy.
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FEBS J The chromatin remodeling protein INO80 contributes to the removal of H2A.Z at the p53-binding site of the p21 gene in response to doxorubicin.
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