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  • KD/KO Validated

INO80 Polyclonal antibody

INO80 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 24819-1-AP

Synonyms

hINO80, INO80, INO80 homolog (S. cerevisiae), INO80A, INOC1, KIAA1259



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24819-1-AP is INO80 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INO80 fusion protein Ag20309
Full Name INO80 homolog (S. cerevisiae)
Calculated molecular weight 1556 aa, 177 kDa
Observed molecular weight 177 kDa, 150 kDa
GenBank accession numberBC146785
Gene symbol INO80
Gene ID (NCBI) 54617
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DNA helicase INO80, also namd as INO80 or hINO80, is a 1,556 amino acid protein, which contains one helicase C-terminal domain, one helicase ATP-binding domain, and one DBINO domain. INO80 co-localizes with PCMA at replication forks during S-phase in the nucleus and belongs to the SNF2 helicase family. INO80 is a component of the chromatin remolding INO80 complex and is widely expressed in many tissues, such as brain,liver and pancreas. INO80 is involved in transcription regulation.

Protocols

Product Specific Protocols
WB protocol for INO80 antibody 24819-1-APDownload protocol
IP protocol for INO80 antibody 24819-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Rep

Beyond autophagy: a novel role for autism-linked Wdfy3 in brain mitophagy.

Authors - Eleonora Napoli
  • KD Validated

Genes Dev

INO80 governs superenhancer-mediated oncogenic transcription and tumor growth in melanoma.

Authors - Bingying Zhou
humanWB

J Allergy Clin Immunol

An inherited immunoglobulin class-switch recombination deficiency associated with a defect in the INO80 chromatin remodeling complex.

Authors - Sven Kracker