INPP4A Polyclonal antibody

INPP4A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 12665-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Type I inositol 3,4-bisphosphate 4-phosphatase, INPP4, INP4A, Inositol polyphosphate-4-phosphatase type I A, Inositol polyphosphate 4-phosphatase type I

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12665-1-AP targets INPP4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INPP4A fusion protein Ag3346 Predict reactive species
Full Name inositol polyphosphate-4-phosphatase, type I, 107kDa
Calculated Molecular Weight 972 aa, 109 kDa
Observed Molecular Weight100-110 kDa
GenBank Accession NumberBC028361
Gene Symbol INPP4A
Gene ID (NCBI) 3631
RRIDAB_3669147
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96PE3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inositol polyphosphate 4 phosphatase (INPP4A) belongs to the group of phosphoinositide phosphatases controlling proliferation, apoptosis, and endosome function by hydrolyzing phosphatidylinositol 3,4-bisphosphate. INPP4A is highly expressed in the central nervous system, which is shown to play a key role in glutamate excitotoxicity and cell proliferation. (PMID: 36653678, PMID: 31938306)

Protocols

Product Specific Protocols
WB protocol for INPP4A antibody 12665-1-APDownload protocol
IHC protocol for INPP4A antibody 12665-1-APDownload protocol
IF protocol for INPP4A antibody 12665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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