INPP4A Polyclonal antibody

INPP4A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12665-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:3.1.3.66, Inositol polyphosphate 4-phosphatase type I, Inositol polyphosphate-4-phosphatase type I A, INP4A, INPP4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis using 12665-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12665-1-AP (INPP4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human intrahepatic cholangiocarcinoma using 12665-1-AP
Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 12665-1-AP (INPP4A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse colon using 12665-1-AP
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12665-1-AP (INPP4A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 12665-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12665-1-AP (INPP4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 12665-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12665-1-AP (INPP4A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of A549 using 12665-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using INPP4A antibody (12665-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, rat brain tissue
Positive IHC detected in	human intrahepatic cholangiocarcinoma tissue, human stomach cancer tissue, mouse colon tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12665-1-AP targets INPP4A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3346 Product name: Recombinant human INPP4A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC028361 Sequence: MTAREHSPRHGARARAMQRASTIDVAADMLGLSLAGNIQDPDEPILEFSLACSELHTPSLDRKPNSFVAVSVTTPPQAFWTKHAQTEIIEGTNNPIFLSSIAFFQDSLINQMTQVKLSVYDVKDRSQGTMYLLGSGTFIVKDLLQDRHHRLHLTLRSAESDRVGNITVIGWQMEEKSDQRPPVTRSVDTVNGRMVLPVDESLTEALGIRSKYASLRKDTLLKSVFGGAICRMYRFPTTDGNHLRILEQMAESVLSLHVPRQFVKLLLEEDAARVCELEELGELSPCWESLRRQIVTQYQTI Predict reactive species
Full Name	inositol polyphosphate-4-phosphatase, type I, 107kDa
Calculated Molecular Weight	972 aa, 109 kDa
Observed Molecular Weight	100-110 kDa
GenBank Accession Number	BC028361
Gene Symbol	INPP4A
Gene ID (NCBI)	3631
RRID	AB_3669147
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96PE3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inositol polyphosphate 4 phosphatase (INPP4A) belongs to the group of phosphoinositide phosphatases controlling proliferation, apoptosis, and endosome function by hydrolyzing phosphatidylinositol 3,4-bisphosphate. INPP4A is highly expressed in the central nervous system, which is shown to play a key role in glutamate excitotoxicity and cell proliferation. (PMID: 36653678, PMID: 31938306)

Protocols

Product Specific Protocols
IF protocol for INPP4A antibody 12665-1-AP	Download protocol
IHC protocol for INPP4A antibody 12665-1-AP	Download protocol
WB protocol for INPP4A antibody 12665-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The INPP4A Polyclonal antibody (Cat# 12665-1-AP) has 1 total citation. It appears in Genome Medicine (IF 10.8), published in 2025. The cited study investigates collateral metabolic vulnerabilities arising from loss of isozyme diversity in breast cancer, placing this antibody's use within the fields of cancer metabolism and phosphoinositide signaling. The application used was Western blot (WB) in human samples.