INPP5A Polyclonal antibody

• Featured Product
• KD/KO Validated

INPP5A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21723-1-AP

Synonyms

Type I inositol 1,4,5-trisphosphate 5-phosphatase, Inositol polyphosphate-5-phosphatase A, EC:3.1.3.56, 5PTASE, 43 kDa inositol polyphosphate 5-phosphatase

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Validation Data Gallery

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  WB analysis of HeLa using 21723-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 21723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21723-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 21723-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 21723-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21723-1-AP (INPP5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, COLO 320 cells, HeLa cells, HepG2 cells, human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

21723-1-AP targets INPP5A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INPP5A fusion protein Ag16380
• Full Name: inositol polyphosphate-5-phosphatase, 40kDa
• Calculated Molecular Weight: 412 aa, 48 kDa
• Observed Molecular Weight: 43-48 kDa
• GenBank Accession Number: BC096280
• Gene Symbol: INPP5A
• Gene ID (NCBI): 3632
• RRID: AB_10734438
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Dev Cell; Functional genetic screen identifies ITPR3/calcium/RELB axis as a driver of colorectal cancer metastatic liver colonization.; Authors - Ryan H Moy ; KD Validated