INPP5A Recombinant antibody

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  WB analysis using 86001-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86001-1-RR (INPP5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86001-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86001-1-RR against Human INPP5A were performed. The affinity constant is below 1 pM.

INPP5A Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86001-1-RR

Clone No.250584C3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

43 kDa inositol polyphosphate 5-phosphatase, 5PTASE, EC:3.1.3.56, Inositol polyphosphate-5-phosphatase A, Type I inositol 1,4,5-trisphosphate 5-phosphatase

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Tested Applications

• Positive WB detected in: HeLa cells, fetal human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86001-1-RR targets INPP5A in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: INPP5A fusion protein Ag16380
• Full Name: inositol polyphosphate-5-phosphatase, 40kDa
• Calculated Molecular Weight: 412 aa, 48 kDa
• GenBank Accession Number: BC096280
• Gene Symbol: INPP5A
• Gene ID (NCBI): 3632
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14642
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INPP5A (Inositol Polyphosphate-5-Phosphatase A) is a membrane-bound type I inositol 1,4,5-trisphosphate 5-phosphatase that is involved in the mobilization of intracellular calcium ions and the regulation of cellular responses to stimuli. It terminates calcium signaling by hydrolyzing inositol 1,4,5-trisphosphate (InsP3) and inositol 1,3,4,5-tetrakisphosphate (InsP4). INPP5A plays important roles in various cellular processes, including cell proliferation, apoptosis, vesicular transport, and insulin signaling. Additionally, INPP5A is crucial for the survival of cerebellar Purkinje cells. In certain cancers, such as cutaneous squamous cell carcinoma (cSCC) and esophageal squamous cell carcinoma (ESCC), the expression levels of INPP5A are closely related to disease progression and prognosis.