INPP5D Polyclonal antibody

INPP5D Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 19694-1-AP

Synonyms

hp51CN, INPP5D, p150Ship, SHIP, SHIP 1, SHIP1, SIP 145

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Validation Data Gallery

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  WB analysis using 19694-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19694-1-AP (INPP5D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Ramos using 19694-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 19694-1-AP (INPP5D Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Ramos using 19694-1-AP

  IP result of anti-INPP5D (IP:19694-1-AP, 4ug; Detection:19694-1-AP 1:300) with Ramos cells lysate 3600 ug.

• 

  IHC staining of human tonsillitis using 19694-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19694-1-AP (INPP5D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19694-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19694-1-AP (INPP5D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19694-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19694-1-AP (INPP5D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19694-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19694-1-AP (INPP5D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Daudi cells, Ramos cells, Raji cells, THP-1 cells
• Positive IP detected in: Ramos cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

19694-1-AP targets INPP5D in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: inositol polyphosphate-5-phosphatase, 145kDa
• Calculated Molecular Weight: 133 kDa
• Observed Molecular Weight: 145 kDa
• GenBank Accession Number: NM_001017915
• Gene Symbol: INPP5D
• Gene ID (NCBI): 3635
• RRID: AB_10667407
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INPP5D, also named as SHIP, SHIP1, SIP-145 and hp51CN, belongs to the inositol-1,4,5-trisphosphate 5-phosphatase family. INPP5D is phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. INPP5D acts as a negative regulator of B-cell antigen receptor signaling. It mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. INPP5D acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. INPP5D regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. It is involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. It is a key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. It modulates FCGR3/CD16-mediated cytotoxicity in NK cells. It mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. INPP5D may also hydrolyze PtdIns(1,3,4,5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6. This antibody is specific to INPP5D.

Publications

Species	Application	Title
human	IHC	Acta Neuropathol Commun; The presubiculum is preserved from neurodegenerative changes in Alzheimer's disease.; Authors - Christina E Murray
human	WB	J Immunol; Systemic Lupus Erythematosus Immune Complexes Increase the Expression of SLAM Family Members CD319 (CRACC) and CD229 (LY-9) on Plasmacytoid Dendritic Cells and CD319 on CD56dim NK Cells.; Authors - Hagberg Niklas N
mouse	WB	Eur J Immunol; Monomer hapten and hapten-specific IgG inhibit mast cell activation evoked by multivalent hapten with different mechanisms.; Authors - Ruriko Suzuki
mouse	WB	Immunol Cell Biol; Mycobacterium tuberculosis Rv1987 protein induces M2 polarization of macrophages through activating the PI3K/Akt1/mTOR signaling pathway.; Authors - Shanshan Sha
human	WB,IHC	Oncol Rep; SHIP1 inhibits cell growth, migration, and invasion in non‑small cell lung cancer through the PI3K/AKT pathway.; Authors - Qiaofen Fu