INPP5J Polyclonal antibody

• Featured Product
• KD/KO Validated

INPP5J Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 21417-1-AP

Synonyms

INPP5, INPP5J, PIB5PA, PIPP

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Validation Data Gallery

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  WB analysis of human heart using 21417-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21417-1-AP (INPP5J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21417-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21417-1-AP (INPP5J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 21417-1-AP

  IP result of anti-INPP5J (IP:21417-1-AP, 4ug; Detection:21417-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human kidney using 21417-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 21417-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 21417-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 21417-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21417-1-AP (INPP5J antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 21417-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 21417-1-AP (INPP5J antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human heart tissue, mouse lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

21417-1-AP targets INPP5J in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INPP5J fusion protein Ag15996
• Full Name: inositol polyphosphate-5-phosphatase J
• Calculated Molecular Weight: 1006 aa, 107 kDa
• Observed Molecular Weight: 107 kDa, 70 kDa
• GenBank Accession Number: BC109288
• Gene Symbol: INPP5J
• Gene ID (NCBI): 27124
• RRID: AB_10859784
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INPP5J(Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A) is also named as PIB5PA, PIPP and belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family. INPP5J is a key signalling molecule that regulates dendritic outgrowth through activation of small GTPase signalling via interaction between FRMPD4 and ARHGEF7. It can control the resting membrane potential through a specific ion-channel-receptor-ligand interaction that brings about a large conformational change, analogous to neurotransmitter activation of ion channels at synapses. (PMID:22595022). This protein has 3 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,IHC	Nat Commun; miR-508 sustains phosphoinositide signalling and promotes aggressive phenotype of oesophageal squamous cell carcinoma.; Authors - Chuyong Lin
human	WB	Biomed Pharmacother; MiR-661 contributed to cell proliferation of human ovarian cancer cells by repressing INPP5J expression.; Authors - Tongyu Zhu
human	WB,IHC,IF	Cell Signal; PELI1 overexpression contributes to pancreatic cancer progression through upregulating ubiquitination-mediated INPP5J degradation; Authors - Wenyang Zhou ; KD Validated