INPP5J Polyclonal antibody

INPP5J Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 29949-1-AP

Synonyms

INPP5, INPP5J, PIB5PA, PIPP

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Validation Data Gallery

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  WB analysis using 29949-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29949-1-AP (INPP5J antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 29949-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using INPP5J antibody (29949-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, MCF-7 cells, mouse heart tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29949-1-AP targets INPP5J in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INPP5J fusion protein Ag29242
• Full Name: inositol polyphosphate-5-phosphatase J
• Calculated Molecular Weight: 1006 aa, 107 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC109288
• Gene Symbol: INPP5J
• Gene ID (NCBI): 27124
• RRID: AB_2918353
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.