Tested Applications
| Positive WB detected in | HEK-293 cells, mouse heart tissue |
| Positive IHC detected in | human kidney tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15098-1-AP targets INPP5K in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7192 Product name: Recombinant human INPP5K protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 81-448 aa of BC004362 Sequence: SPLSFIKVSHVRMQGILLLVFAKYQHLPYIQILSTKSTPTGLFGYWGNKGGVNICLKLYGYYVSIINCHLPPHISNNYQRLEHFDRILEMQNCEGRDIPNILDHDLIIWFGDMNFRIEDFGLHFVRESIKNRCYGGLWEKDQLSIAKKHDPLLREFQEGRLLFPPTYKFDRNSNDYDTSEKKRKPAWTDRILWRLKRQPCAGPDTPIPPASHFSLSLRGYSSHMTYGISDHKPVSGTFDLELKPLVSAPLIVLMPEDLWTVENDMMVSYSSTSDFPSSPWDWIGLYKVGLRDVNDYVSYAWVGDSKVSCSDNLNQVYIDISNIPTTEDEFLLCYYSNSLRSVVGISRPFQIPPGSLREDPLGEAQPQI Predict reactive species |
| Full Name | inositol polyphosphate-5-phosphatase K |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 45-51 kDa |
| GenBank Accession Number | BC004362 |
| Gene Symbol | INPP5K |
| Gene ID (NCBI) | 51763 |
| RRID | AB_2126365 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BT40 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for INPP5K antibody 15098-1-AP | Download protocol |
| IHC protocol for INPP5K antibody 15098-1-AP | Download protocol |
| WB protocol for INPP5K antibody 15098-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The INPP5K Polyclonal antibody (Cat# 15098-1-AP) has 4 citations published in Cell Chem Biol, Exp Neurol, Front Mol Neurosci, and BMC Cancer. Research fields span inositol pyrophosphate interactome profiling, m6A-mediated rRNA modification in traumatic brain injury, INPP5K knockdown effects on neuronal differentiation, and phosphoinositide metabolism in cervical cancer prognosis.
| Species | Application | Title |
|---|---|---|
Cell Chem Biol Triplexed Affinity Reagents to Sample the Mammalian Inositol Pyrophosphate Interactome. | ||
Exp Neurol METTL5-mediated 18S rRNA m6A modification promotes corticospinal tract sprouting after unilateral traumatic brain injury | ||
BMC Cancer Phosphoinositide metabolism-related prognostic genes and their prognostic value in cervical cancer: an investigation based on transcriptome, single-cell analyses and verification. |
Reviews
What customers say
The single review for this product reports a 4-star rating from a Western Blot application. The reviewer used a 1/1000 dilution on Cos7 cells and noted that the antibody detected transfected GFP-tagged INPP5K well, with the membrane incubated overnight in the fridge. Overall, the feedback is positive, indicating reliable performance for detecting the target protein under standard WB conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tim (Verified Customer) (06-28-2021) | Detected transfected GFP-tagged INPP5K in Cos7 cells well. Antibody incubated with membrane overnight in fridge.
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