INPP5K Polyclonal antibody

INPP5K Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 15098-1-AP

Synonyms

Phosphatidylinositol-4,5-bisphosphate 5-phosphatase, Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase, Inositol polyphosphate 5-phosphatase K, EC:3.1.3.86, EC:3.1.3.56

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Validation Data Gallery

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  WB analysis of HEK-293 using 15098-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15098-1-AP (INPP5K antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 15098-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15098-1-AP (INPP5K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15098-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15098-1-AP (INPP5K antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15098-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15098-1-AP (INPP5K antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15098-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15098-1-AP (INPP5K antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15098-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15098-1-AP (INPP5K antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HEK-293 using 15098-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15098-1-AP (INPP5K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse heart tissue
• Positive IHC detected in: human kidney tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

15098-1-AP targets INPP5K in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INPP5K fusion protein Ag7192
• Full Name: inositol polyphosphate-5-phosphatase K
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 45-51 kDa
• GenBank Accession Number: BC004362
• Gene Symbol: INPP5K
• Gene ID (NCBI): 51763
• RRID: AB_2126365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Chem Biol; Triplexed Affinity Reagents to Sample the Mammalian Inositol Pyrophosphate Interactome.; Authors - David Furkert
human,mouse	WB,IF	Front Mol Neurosci; Knockdown of INPP5K compromises the differentiation of N2A cells; Authors - Annamaria Manzolillo
mouse	WB	Exp Neurol; METTL5-mediated 18S rRNA m6A modification promotes corticospinal tract sprouting after unilateral traumatic brain injury; Authors - Zhenpeng Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tim (Verified Customer) (06-28-2021)

Detected transfected GFP-tagged INPP5K in Cos7 cells well. Antibody incubated with membrane overnight in fridge.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Cos7

INPP5K Antibody Western Blot validation (1/1000 dilution) in Cos7 (Cat no:15098-1-AP)