Tested Applications
| Positive WB detected in | MCF-7 cells, HL-60 cells, rat testis tissue, HeLa cells, Jurkat cells, MDA-MB-231 cells, mouse kidney tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse pancreas tissue, rat pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15271-1-AP targets INTS10 in WB, IHC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7383 Product name: Recombinant human INTS10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 37-382 aa of BC001915 Sequence: LLKVTEQCFNTLERSEMLLLLLRRFPETVVQHGVGLGEALLEAETIEEQESPVNCFRKLFVCDVLPLIINNHDVRLPANLLYKYLNKAAEFYINYVTRSTQIENQHQGAQDTSDLMSPSKRSSQKYIIEGLTEKSSQIVDPWERLFKILNVVGMRCEWQMDKGRRSYGDILHRMKDLCRYMNNFDSEAHAKYKNQVVYSTMLVFFKNAFQYVNSIQPSLFQGPNAPSQVPLVLLEDVSNVYGDVEIDRNKHIHKKRKLAEGREKTMSSDDEDCSAKGRNRHIVVNKAELANSTEVLESFKLARESWELLYSLEFLDKEFTRICLAWKTDTWLWLRIFLTDMIIYQG Predict reactive species |
| Full Name | integrator complex subunit 10 |
| Calculated Molecular Weight | 82 kDa |
| Observed Molecular Weight | 70-82 kDa |
| GenBank Accession Number | BC001915 |
| Gene Symbol | INTS10 |
| Gene ID (NCBI) | 55174 |
| RRID | AB_2127260 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NVR2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
INTS10 is a subunit of the Integrator complex, which associates with the C-terminal domain of RNA polymerase II large subunit. INTS10, Asunder/INTS13 and INTS14 form a separable, functional Integrator module.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for INTS10 antibody 15271-1-AP | Download protocol |
| IP protocol for INTS10 antibody 15271-1-AP | Download protocol |
| WB protocol for INTS10 antibody 15271-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The INTS10 Polyclonal Antibody (Cat# 15271-1-AP) has 15 citations published in journals including Nature, Nature Communications, Molecular Cell, PNAS, Cell Reports, eLife, FASEB Journal, International Journal of Molecular Sciences, BMC Pregnancy and Childbirth, and Protein Expression and Purification. Research fields covered include transcription regulation, Integrator complex biology, RNA processing, viral replication, and clinical biomarker analysis.
| Species | Application | Title |
|---|---|---|
Nat Commun DSIF and NELF interact with Integrator to specify the correct post-transcriptional fate of snRNA genes. | ||
Nat Commun INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module.
| ||
Nat Commun INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex | ||
Mol Cell Integrator endonuclease drives promoter-proximal termination at all RNA polymerase II-transcribed loci | ||
Mol Cell PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII |
