INTS3 Polyclonal antibody

• Featured Product
• KD/KO Validated

INTS3 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 16620-1-AP

Synonyms

Sensor of ssDNA subunit A, Sensor of single-strand DNA complex subunit A, Integrator complex subunit 3, Int3, C1orf60

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Validation Data Gallery

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  WB analysis using 16620-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16620-1-AP (INTS3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16620-1-AP

  WB result of INTS3 antibody (16620-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INTS3 transfected HEK-293 cells.

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  IP experiment of HEK-293 using 16620-1-AP

  IP result of anti-INTS3 (IP:16620-1-AP, 4ug; Detection:16620-1-AP 1:1000) with HEK-293 cells lysate 1360 ug.

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  IHC staining of human ovary cancer using 16620-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16620-1-AP (INTS3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• ChIP: See 2 publications below

Product Information

16620-1-AP targets INTS3 in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INTS3 fusion protein Ag9917
• Full Name: integrator complex subunit 3
• Calculated Molecular Weight: 118 kDa
• Observed Molecular Weight: 118 kDa
• GenBank Accession Number: BC025254
• Gene Symbol: INTS3
• Gene ID (NCBI): 65123
• RRID: AB_2127274
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INTS3 is a member of the integrator complex characterised as an RNA polymerase II (RNAPII) C-terminal domain binding factor involved in the processing of small nuclear RNAs (snRNAs) (PMID: 16239144). INTS3 was overexpressed in colorectal cancer (CRC) patients and tumour cell lines and inhibited CRC apoptosis by promoting the degradation of pro-apoptotic gene transcripts (PMID: 38665208).

Publications

Species	Application	Title
mouse	WB	Science; Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.; Authors - Hai Zheng
human	WB	Cell; The PP2A-Integrator-CDK9 axis fine-tunes transcription and can be targeted therapeutically in cancer.; Authors - Stephin J Vervoort
mouse	WB	Mol Cell; PGC-1α senses the CBC of pre-mRNA to dictate the fate of promoter-proximally paused RNAPII; Authors - Xavier Rambout
human	WB	Mol Cell; Integrator Recruits Protein Phosphatase 2A to Prevent Pause Release and Facilitate Transcription Termination.; Authors - Kai-Lieh Huang
human	chIP	Mol Cell; Deregulated Expression of Mammalian lncRNA through Loss of SPT6 Induces R-Loop Formation, Replication Stress, and Cellular Senescence.; Authors - Takayuki Nojima
human	WB	Nat Commun; INTS13 variants causing a recessive developmental ciliopathy disrupt assembly of the Integrator complex; Authors - Lauren G. Mascibroda