INTS4 Polyclonal antibody

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INTS4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16130-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Int4, MST093, MSTP093

• 

  WB analysis using 16130-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16130-1-AP (INTS4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 16130-1-AP

  WB result of INTS4 antibody (16130-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-INTS4 transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 16130-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16130-1-AP (INTS4 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16130-1-AP targets INTS4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9116

  Product name: Recombinant human INTS4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-331 aa of BC009995

  Sequence: MAAHLKKRVYEEFTKVVQPQEEIATKKLRLTKPSKSAALHIDLCKATSPADALQYLLQFARKPVEAESVEGVVRILLEHYYKENDPSVRLKIASLLGLLSKTAGFSPDCIMDDAINILQNEKSHQVLAQLLDTLLAIGTKLPENQAIQMRLVDVACKHLTDTSHGVRNKCLQLLGNLGSLEKSVTKDAEGLAARDVQKIIGDYFSDQDPRVRTAAIKAMLQLHERGLKLHQTIYNQACKLLSDDYEQVRSAAVQLIWVVSQLYPESIVPIPSSNEEIRLVDDAFGKICHMVSDGSWVVRVQAAKLLGSMEQVSSHFLEQTLDKKLMSDLRR

• Full Name: integrator complex subunit 4
• Calculated Molecular Weight: 963aa,108 kDa; 505aa,57 kDa
• Observed Molecular Weight: 108 kDa
• GenBank Accession Number: BC009995
• Gene Symbol: INTS4
• Gene ID (NCBI): 92105
• RRID: AB_2296175
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96HW7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrator complex subunit 4 (INTS4), a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. INTS4 is a specific and conserved interaction partner of INTS9/11 heterodimer that does not interact with either subunit individually (PMID: 29471365). INTS4 plays a role in achieving a compact nuclease/pseudonuclease configuration resembling that observed for CPSF73/100 in the histone-processing machinery (PMID: 33548203).

Publications

What published studies show

The INTS4 Polyclonal antibody (Cat# 16130-1-AP) has 2 citations. These appear in Science Bulletin (2026) and Molecular Cell (2025). The research fields cover transcription elongation and sequential verification of transcription by the Integrator complex, with one study specifically examining how GABPA recruits the Integrator endonuclease complex to promote transcription elongation.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Sci Bull (Beijing); GABPA recruits the Integrator endonuclease complex to promote transcription elongation.; Authors - Hang Yang
human	WB	Mol Cell; Sequential verification of transcription by Integrator and Restrictor.; Authors - Chris Estell