INTS7 Polyclonal antibody

INTS7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17054-1-AP

Synonyms

C1orf73, INT7, integrator complex subunit 7, INTS7

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Validation Data Gallery

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  WB analysis of HEK-293 using 17054-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17054-1-AP (INTS7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17054-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17054-1-AP (INTS7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 17054-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17054-1-AP (INTS7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 17054-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17054-1-AP (INTS7 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of SH-SY5Y using 17054-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 17054-1-AP (INTS7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

17054-1-AP targets INTS7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INTS7 fusion protein Ag10755
• Full Name: integrator complex subunit 7
• Calculated Molecular Weight: 948 aa, 105 kDa
• Observed Molecular Weight: 107 kDa
• GenBank Accession Number: BC020523
• Gene Symbol: INTS7
• Gene ID (NCBI): 25896
• RRID: AB_2127393
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INTS7 is a subunit of the integrator complex. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Plays a role in DNA damage response (DDR) signaling during the S phase (PMID: 21659603).

Publications

Species	Application	Title
human	IHC	Int Immunopharmacol; Overexpression and diagnostic significance of INTS7 in lung adenocarcinoma and its effects on tumor microenvironment.; Authors - Xiang Li
mouse	WB,IF,IP	Front Physiol; INTS7-ABCD3 Interaction Stimulates the Proliferation and Osteoblastic Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells by Suppressing Oxidative Stress.; Authors - Yubo Liu
mouse	WB,CoIP	PeerJ; BAG3 regulates bone marrow mesenchymal stem cell proliferation by targeting INTS7; Authors - Yubo Liu
human	WB,IHC	Sci Rep; The pro-tumor activity of INTS7 on lung adenocarcinoma via inhibiting immune infiltration and activating p38MAPK pathway; Authors - Xiang Li