INTS7 Polyclonal antibody

INTS7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17054-1-AP

Synonyms

C1orf73, INT7, integrator complex subunit 7, INTS7



Tested Applications

Positive WB detected inHEK-293 cells, A431 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17054-1-AP targets INTS7 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen INTS7 fusion protein Ag10755
Full Name integrator complex subunit 7
Calculated Molecular Weight 948 aa, 105 kDa
Observed Molecular Weight 107 kDa
GenBank Accession NumberBC020523
Gene Symbol INTS7
Gene ID (NCBI) 25896
RRIDAB_2127393
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INTS7 is a subunit of the integrator complex. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Plays a role in DNA damage response (DDR) signaling during the S phase (PMID: 21659603).

Protocols

Product Specific Protocols
WB protocol for INTS7 antibody 17054-1-APDownload protocol
IHC protocol for INTS7 antibody 17054-1-APDownload protocol
IF protocol for INTS7 antibody 17054-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int Immunopharmacol

Overexpression and diagnostic significance of INTS7 in lung adenocarcinoma and its effects on tumor microenvironment.

Authors - Xiang Li
mouseWB,IF,IP

Front Physiol

INTS7-ABCD3 Interaction Stimulates the Proliferation and Osteoblastic Differentiation of Mouse Bone Marrow Mesenchymal Stem Cells by Suppressing Oxidative Stress.

Authors - Yubo Liu
mouseWB,CoIP

PeerJ

BAG3 regulates bone marrow mesenchymal stem cell proliferation by targeting INTS7

Authors - Yubo Liu
humanWB,IHC

Sci Rep

The pro-tumor activity of INTS7 on lung adenocarcinoma via inhibiting immune infiltration and activating p38MAPK pathway

Authors - Xiang Li