INTS9 Polyclonal antibody

INTS9 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 11657-1-AP

Synonyms

RC 74, INTS 9, Int9, CPSF2L, CPSF2

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Validation Data Gallery

• 

  WB analysis of Jurkat using 11657-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11657-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11657-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11657-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11657-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11657-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11657-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11657-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11657-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11657-1-AP (INTS9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11657-1-AP

  IP result of anti-INTS9 (IP:11657-1-AP, 4ug; Detection:11657-1-AP 1:1000) with HeLa cells lysate 880ug.

• 

  IHC staining of human stomach using 11657-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 11657-1-AP (INTS9 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IP: See 2 publications below

Product Information

11657-1-AP targets INTS9 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: INTS9 fusion protein Ag2226
• Full Name: integrator complex subunit 9
• Calculated Molecular Weight: 658 aa, 74 kDa
• Observed Molecular Weight: 74 kDa
• GenBank Accession Number: BC025267
• Gene Symbol: INTS9
• Gene ID (NCBI): 55756
• RRID: AB_2127514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; Assembly mechanism of Integrator's RNA cleavage module; Authors - Kevin Sabath
human	WB	Nat Commun; INTS10-INTS13-INTS14 form a functional module of Integrator that binds nucleic acids and the cleavage module.; Authors - Kevin Sabath
human	WB	Protein Expr Purif; CRISPR-Cas9 mediated genetic engineering for the purification of the endogenous integrator complex from mammalian cells.; Authors - David Baillat
human	WB,IP	Mol Cell; Integrator facilitates RNAPII removal to prevent transcription-replication collisions and genome instability; Authors - Rahul Bhowmick
human	WB,IP	Mol Cell; Cytoplasmic binding partners of the Integrator endonuclease INTS11 and its paralog CPSF73 are required for their nuclear function; Authors - Min-Han Lin