IP6K1 Polyclonal antibody

IP6K1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12057-2-AP

Synonyms

IHPK1, InsP6 kinase 1, IP6K1, KIAA0263, PiUS

---

Validation Data Gallery

• 

  WB analysis using 12057-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12057-2-AP (IP6K1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12057-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12057-2-AP (IP6K1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12057-2-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12057-2-AP (IP6K1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 12057-2-AP

  IP result of anti-IP6K1 (IP:12057-2-AP, 4ug; Detection:12057-2-AP 1:300) with mouse kidney tissue lysate 3000 ug.

• 

  IHC staining of human lung cancer using 12057-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12057-2-AP (IP6K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12057-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12057-2-AP (IP6K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12057-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12057-2-AP (IP6K1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12057-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12057-2-AP (IP6K1 antibody) at dilution of 1:50 (under 10x lens).

---

Tested Applications

• Positive WB detected in: A375 cells, human liver tissue, human kidney tissue, Hela cells, HepG2 cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human colon cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12057-2-AP targets IP6K1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IP6K1 fusion protein Ag2687
• Full Name: inositol hexakisphosphate kinase 1
• Calculated Molecular Weight: 441 aa, 50 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC012944
• Gene Symbol: IP6K1
• Gene ID (NCBI): 9807
• RRID: AB_2127529
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
pig	WB	Pediatr Res; Preterm birth alters the feeding-induced activation of Akt signaling in the muscle of neonatal piglets; Authors - Agus Suryawan