IP6K2 Polyclonal antibody

IP6K2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13155-1-AP

Synonyms

IHPK2, InsP6 kinase 2, IP6K2, P(i) uptake stimulator, PiUS

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Validation Data Gallery

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  WB analysis of human adrenal gland using 13155-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 13155-1-AP (IP6K2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13155-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13155-1-AP (IP6K2 antibody) at dilution of 1:1800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 13155-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13155-1-AP (IP6K2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 13155-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13155-1-AP (IP6K2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human adrenal gland tissue, human heart tissue, K-562 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3600
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13155-1-AP targets IP6K2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IP6K2 fusion protein Ag3786
• Full Name: inositol hexakisphosphate kinase 2
• Calculated Molecular Weight: 426 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC019694
• Gene Symbol: IP6K2
• Gene ID (NCBI): 51447
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IP6K2(Inositol hexakisphosphate kinase 2) is also named as IHPK2, PiUS and belongs to the inositol phosphokinase (IPK) family. It converts inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5) and and mediates apoptotic cell death via its translocation from the nucleus to the cytoplasm(PMID:21652713). IP6K2 regulates TP53 and protein kinase AKT signaling, and its aberrant increase in cells has been implicated in apoptosis and INS resistance(PMID:21262846). It has 6 isoforms produced by alternative splicing. This antibody recognizes the others except the 8 kDa isoform of IP6K2.