IPO11 Polyclonal antibody

• Featured Product
• KD/KO Validated

IPO11 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 14403-1-AP

Synonyms

Imp11, importin 11, IPO11, Ran binding protein 11, RanBP11

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Validation Data Gallery

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  WB analysis of human testis using 14403-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14403-1-AP (IPO11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 14403-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14403-1-AP (IPO11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 14403-1-AP

  IP result of anti-IPO11 (IP:14403-1-AP, 4ug; Detection:14403-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of human lung cancer using 14403-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14403-1-AP (IPO11 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung cancer using 14403-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 14403-1-AP (IPO11 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 14403-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14403-1-AP (IPO11 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14403-1-AP targets IPO11 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IPO11 fusion protein Ag5659
• Full Name: importin 11
• Calculated Molecular Weight: 113 kDa
• Observed Molecular Weight: 112 kDa
• GenBank Accession Number: BC033776
• Gene Symbol: IPO11
• Gene ID (NCBI): 51194
• RRID: AB_2296214
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Importins are members of a family of transport receptors (karyopherins) that mediate the nucleocytoplasmic transport of protein and RNA cargoes. Importin-11, is a nuclear import receptor for the ubiquitin-conjugating enzyme, UbcM2.

Publications

Species	Application	Title
human,mouse	WB,IF	Mol Cancer; Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling.; Authors - Yang Gu
human	WB	Genomics Proteomics Bioinformatics; Integrative Multi-omics Landscape of Non-structural Protein 3 of Severe Acute Respiratory Syndrome Coronaviruses.; Authors - Ruona Shi
human	WB,IHC	Urol Oncol; Importin-11 overexpression promotes the migration, invasion, and progression of bladder cancer associated with the deregulation of CDKN1A and THBS1.; Authors - Junjie Zhao ; KD Validated
mouse	WB	Int J Med Sci; Importin-11 is Essential for Normal Embryonic Development in Mice.; Authors - Ju-Young Lee ; KO Validated
		Oncotarget; Whole-exome sequencing of muscle-invasive bladder cancer identifies recurrent copy number variation in IPO11 and prognostic significance of importin-11 overexpression on poor survival.; Authors - Junjie Zhao