Tested Applications
| Positive WB detected in | HeLa cells, human brain tissue, mouse brain tissue, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11679-1-AP targets IPO4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2281 Product name: Recombinant human IPO4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 401-787 aa of BC003690 Sequence: MESVFEEVFKLLECPHLNVRKAAHEALGQFCCALHKACQSCPSEPNTAALQAALARVVPSYMQAVNRERERQVVMAVLEALTGVLRSCGTLTLKPPGRLAELCGVLKAVLQRKTACQDTDEEEEEEDDDQAEYDAMLLEHAGEAIPALAAAAGGDSFAPFFAGFLPLLVCKTKQGCTVAEKSFAVGTLAETIQGLGAASAQFVSRLLPVLLSTAQEADPEVRSNAIFGMGVLAEHGGHPAQEHFPKLLGLLFPLLARERHDRVRDNICGALARLLMASPTRKPEPQVLAALLHALPLKEDLEEWVTIGRLFSFLYQSSPDQVIDVAPELLRICSLILADNKIPPDTKAALLLLLTFLAKQHTDSFQAALGSLPVDKAQELQAVLGLS Predict reactive species |
| Full Name | importin 4 |
| Calculated Molecular Weight | 1081 aa, 118 kDa |
| Observed Molecular Weight | 118 kDa |
| GenBank Accession Number | BC003690 |
| Gene Symbol | IPO4 |
| Gene ID (NCBI) | 79711 |
| RRID | AB_2127677 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TEX9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Importin‐4 (IPO4) belongs to the importin β family which directs the cargo to pass through the nuclear pore complex via transient interaction with nucleoporins. IPO4 is responsible for transporting histones H3 and H4 into the nucleus for chromatin assembly. Recently IPO4 has been reported to contribute to gastric cancer progression and poor prognosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IPO4 antibody 11679-1-AP | Download protocol |
| IHC protocol for IPO4 antibody 11679-1-AP | Download protocol |
| IP protocol for IPO4 antibody 11679-1-AP | Download protocol |
| WB protocol for IPO4 antibody 11679-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-IPO4 antibody (Cat# 11679-1-AP) has 16 total citations published in journals including Cell Research, Molecular Cell, PNAS, Science Advances, Cell Reports, Cancer Cell International, Cell Signaling, Genomics Proteomics Bioinformatics, Medical Oncology, Journal of Cellular Biochemistry, PLoS ONE, Gene, and Biochemical and Biophysical Research Communications. Research fields span cancer biology (hepatocellular, glioma, bladder, ovarian, gastric), nuclear protein import, DNA damage response, viral infection, cilia targeting, innate immunity, and nucleotide metabolism.
| Species | Application | Title |
|---|---|---|
Cell Res Neddylation of PTEN regulates its nuclear import and promotes tumor development. | ||
Mol Cell Rate-limiting enzymes in nucleotide metabolism synchronize nucleotide biosynthesis and chromatin formation. | ||
J Exp Clin Cancer Res Transketolase (TKT) activity and nuclear localization promote hepatocellular carcinoma in a metabolic and a non-metabolic manner. | ||
Sci Adv The nuclear import receptor importin-7 targets HPV from the Golgi to the nucleus to promote infection.
| ||
Proc Natl Acad Sci U S A ARL13B, PDE6D, and CEP164 form a functional network for INPP5E ciliary targeting. | ||
Proc Natl Acad Sci U S A CCAAT/enhancer binding protein delta (C/EBPdelta, CEBPD)-mediated nuclear import of FANCD2 by IPO4 augments cellular response to DNA damage. |
