Tested Applications
| Positive WB detected in | HeLa cells, U-251 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28289-1-AP targets IPO7 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27257 Product name: Recombinant human IPO7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 879-1016 aa of NM_006391.2 Sequence: MHAEHENDSDDDDEAEDDDETEELGSDEDDIDEDGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQTIQNRNPVWYQALTHGLNEEQRKQLQDIATLADQRRAAHESKMIEKHGG Predict reactive species |
| Full Name | importin 7 |
| Calculated Molecular Weight | 120 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | NM_006391.2 |
| Gene Symbol | IPO7 |
| Gene ID (NCBI) | 10527 |
| RRID | AB_2881106 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95373 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for IPO7 antibody 28289-1-AP | Download protocol |
| WB protocol for IPO7 antibody 28289-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Importin-7 antibody (Cat# 28289-1-AP) has 12 citations across journals including Nature Communications, Nature Chemical Biology, Science Advances, Cell Death & Disease, Cell Reports, NPJ Regenerative Medicine, Journal of Cell Biology, iScience, Molecular Immunology, and Cells Development. Research fields span nuclear import/transport, mechanobiology, cancer progression, neurodegeneration (C9orf72/FMR1), inflammatory signaling, and developmental biology.
| Species | Application | Title |
|---|---|---|
Nat Commun Mechanical control of nuclear import by Importin-7 is regulated by its dominant cargo YAP. | ||
Sci Adv The nuclear import receptor importin-7 targets HPV from the Golgi to the nucleus to promote infection.
| ||
Sci Adv Hyperwrinkling of the nuclear lamina is associated with attenuated mechanosensitivity in giant nucleated cancer cells. | ||
Cell Death Dis Nuclear translocation of SLC25A10 isoform 3 promotes chemoresistance in HCC cells via CEBPB/BCL2A1 signaling.
| ||
Cell Rep Gasdermin C reprograms metabolism through the CAMKK2-AMPK axis to promote lung adenocarcinoma progression and radioresistance. |
Reviews
What customers say
One reviewer (María García, Feb 2022) rated this antibody 4 out of 5 stars. She used it for both Western Blot at 1:1000 dilution and Immunofluorescence at 1:100 in permeabilized RPE-1/MSC cells, reporting that it worked well for both applications. She also confirmed its specificity through siRNA validation. Overall, the feedback is positive, highlighting reliable performance across multiple techniques.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
María (Verified Customer) (02-08-2022) | Works fine for WB (1:1000) and IF in permeabilized cells (1:100). Validated with siRNA
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